TY - JOUR
T1 - Serological Diagnosis of Infective endocarditis
AU - Lang, S
AU - Watkin, Richard
AU - Allen, F
AU - Harper, LV
AU - Lambert, PA
AU - Littler, William
AU - Elliott, Thomas
PY - 2004/1/1
Y1 - 2004/1/1
N2 - OBJECTIVES: Establishing the diagnosis of infective endocarditis (IE) can be difficult when blood cultures remain sterile or echocardiography is inconclusive. Staphylococcus aureus is a common aetiological microorganism in IE and is associated with severe valvular destruction and increased mortality. Early diagnosis using culture and antibiotic independent tests would be preferable to allow prompt antibiotic administration. We have developed and evaluated 2 serological assays for the rapid identification of a staphylococcal aetiology in infective endocarditis. The assays measure IgG against whole cells of S. aureus and IgG against lipid S, a novel extracellular antigen released by Gram-positive microorganisms. METHODS: Serum was collected from 130 patients with IE and 94 control patients. IgG against whole cells of S. aureus and against lipid S was measured by enzyme linked immunosorbent assay (ELISA). RESULTS: Anti-lipid S IgG titres were higher in IE caused by Gram-positive microorganisms than in controls (p
AB - OBJECTIVES: Establishing the diagnosis of infective endocarditis (IE) can be difficult when blood cultures remain sterile or echocardiography is inconclusive. Staphylococcus aureus is a common aetiological microorganism in IE and is associated with severe valvular destruction and increased mortality. Early diagnosis using culture and antibiotic independent tests would be preferable to allow prompt antibiotic administration. We have developed and evaluated 2 serological assays for the rapid identification of a staphylococcal aetiology in infective endocarditis. The assays measure IgG against whole cells of S. aureus and IgG against lipid S, a novel extracellular antigen released by Gram-positive microorganisms. METHODS: Serum was collected from 130 patients with IE and 94 control patients. IgG against whole cells of S. aureus and against lipid S was measured by enzyme linked immunosorbent assay (ELISA). RESULTS: Anti-lipid S IgG titres were higher in IE caused by Gram-positive microorganisms than in controls (p
UR - http://www.scopus.com/inward/record.url?scp=33749518498&partnerID=8YFLogxK
U2 - 10.1016/j.jinf.2005.12.009
DO - 10.1016/j.jinf.2005.12.009
M3 - Article
C2 - 16443276
SN - 1532-2742
VL - in press
SP - 301
EP - 307
JO - Journal of Infection
JF - Journal of Infection
ER -