Septin-mediated plant cell invasion by the rice blast fungus, Magnaporthe oryzae

Research output: Contribution to journalArticle

Authors

  • Yasin F Dagdas
  • Kae Yoshino
  • Gulay Dagdas
  • Lauren S Ryder
  • Gero Steinberg
  • Nicholas J Talbot

Colleges, School and Institutes

Abstract

To cause rice blast disease, the fungus Magnaporthe oryzae develops a pressurized dome-shaped cell called an appressorium, which physically ruptures the leaf cuticle to gain entry to plant tissue. Here, we report that a toroidal F-actin network assembles in the appressorium by means of four septin guanosine triphosphatases, which polymerize into a dynamic, hetero-oligomeric ring. Septins scaffold F-actin, via the ezrin-radixin-moesin protein Tea1, and phosphatidylinositide interactions at the appressorium plasma membrane. The septin ring assembles in a Cdc42- and Chm1-dependent manner and forms a diffusion barrier to localize the inverse-bin-amphiphysin-RVS-domain protein Rvs167 and the Wiskott-Aldrich syndrome protein Las17 at the point of penetration. Septins thereby provide the cortical rigidity and membrane curvature necessary for protrusion of a rigid penetration peg to breach the leaf surface.

Details

Original languageEnglish
Pages (from-to)1590-5
Number of pages6
JournalScience
Volume336
Issue number6088
Publication statusPublished - 22 Jun 2012

Keywords

  • Actin Cytoskeleton, Actins, Cell Membrane, Diffusion, Fungal Proteins, Magnaporthe, Microfilament Proteins, Mutation, Oryza, Phosphatidylinositols, Plant Diseases, Plant Leaves, Protein Interaction Domains and Motifs, Recombinant Fusion Proteins, Septins, cdc42 GTP-Binding Protein