Separation of cis and trans isomers of polyproline by FAIMS mass spectrometry
Research output: Contribution to journal › Article › peer-review
Colleges, School and Institutes
High field asymmetric waveform ion mobility spectrometry (FAIMS) is well-established as a tool for separating peptide isomers (sequence inversions and post-translationally modified localization variants). Here, we demonstrate the FAIMS is able to differentiate cis and trans isomers of polyproline. Polyproline assumes an all-cis conformation—the PPI helix—in 1-propanol, and an all-trans conformation—the PPII helix—in aqueous solutions. Differentiation of these conformers may be achieved both through use of a cylindrical FAIMS device and a miniaturized ultrahigh field planar FAIMS device.
|Journal||Journal of the American Society for Mass Spectrometry|
|Early online date||4 Oct 2016|
|Publication status||Published - Dec 2016|