Separase, securin and Rad21 in neural cell growth

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Separase, securin and Rad21 in neural cell growth. / Pemberton, Helen; Franklyn, Jayne; Boelaert, Kristien; Chan, Shiao-yng; Kim, Dae; Kim, C; Cheng, SY; Kilby, Mark; McCabe, Christopher.

In: Journal of Cellular Physiology, Vol. 213, No. 1, 01.10.2007, p. 45-53.

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@article{df344d3434c14b37a2a6862993d90aec,
title = "Separase, securin and Rad21 in neural cell growth",
abstract = "The key mitotic regulator securin is expressed at low levels in fetal brain compared with adult, and modulates the proliferation of human embryonic neuronal N-Tera2 (NT2) cells. We now examine the function and expression of securin's interacting partner separase, along with Rad21, the functional component of cohesin, which is cleaved by separase following interaction with securin. In contrast to securin, the cleaved forms of separase and Rad21 were highly expressed in human fetal cerebral cortex compared with adult. In a murine model of absent securin expression - the PTTG knock-out mouse - separase and Rad21 were over-expressed in multiple brain regions. In addition, cDNA array analysis of other key mitotic regulators additionally identified cyclin C and sestrin 2 to be induced in the brains of securin-null mice compared with wild type. Further, Rad21 mRNA expression was highly correlated with that of securin, separase, cyclin C and sestrin 2 in fetal brains. In embryonic neuronal NT2 cells, siRNA repression of separase failed to significantly alter cell turnover, whereas repression of securin expression resulted in increased levels of the activated forms of Rad21 and separase, and promoted cell proliferation. Our data suggest that the co-ordinated expression of separase, securin and Rad21 is fundamental for the developing brain.",
author = "Helen Pemberton and Jayne Franklyn and Kristien Boelaert and Shiao-yng Chan and Dae Kim and C Kim and SY Cheng and Mark Kilby and Christopher McCabe",
year = "2007",
month = oct,
day = "1",
doi = "10.1002/jcp.21086",
language = "English",
volume = "213",
pages = "45--53",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley",
number = "1",

}

RIS

TY - JOUR

T1 - Separase, securin and Rad21 in neural cell growth

AU - Pemberton, Helen

AU - Franklyn, Jayne

AU - Boelaert, Kristien

AU - Chan, Shiao-yng

AU - Kim, Dae

AU - Kim, C

AU - Cheng, SY

AU - Kilby, Mark

AU - McCabe, Christopher

PY - 2007/10/1

Y1 - 2007/10/1

N2 - The key mitotic regulator securin is expressed at low levels in fetal brain compared with adult, and modulates the proliferation of human embryonic neuronal N-Tera2 (NT2) cells. We now examine the function and expression of securin's interacting partner separase, along with Rad21, the functional component of cohesin, which is cleaved by separase following interaction with securin. In contrast to securin, the cleaved forms of separase and Rad21 were highly expressed in human fetal cerebral cortex compared with adult. In a murine model of absent securin expression - the PTTG knock-out mouse - separase and Rad21 were over-expressed in multiple brain regions. In addition, cDNA array analysis of other key mitotic regulators additionally identified cyclin C and sestrin 2 to be induced in the brains of securin-null mice compared with wild type. Further, Rad21 mRNA expression was highly correlated with that of securin, separase, cyclin C and sestrin 2 in fetal brains. In embryonic neuronal NT2 cells, siRNA repression of separase failed to significantly alter cell turnover, whereas repression of securin expression resulted in increased levels of the activated forms of Rad21 and separase, and promoted cell proliferation. Our data suggest that the co-ordinated expression of separase, securin and Rad21 is fundamental for the developing brain.

AB - The key mitotic regulator securin is expressed at low levels in fetal brain compared with adult, and modulates the proliferation of human embryonic neuronal N-Tera2 (NT2) cells. We now examine the function and expression of securin's interacting partner separase, along with Rad21, the functional component of cohesin, which is cleaved by separase following interaction with securin. In contrast to securin, the cleaved forms of separase and Rad21 were highly expressed in human fetal cerebral cortex compared with adult. In a murine model of absent securin expression - the PTTG knock-out mouse - separase and Rad21 were over-expressed in multiple brain regions. In addition, cDNA array analysis of other key mitotic regulators additionally identified cyclin C and sestrin 2 to be induced in the brains of securin-null mice compared with wild type. Further, Rad21 mRNA expression was highly correlated with that of securin, separase, cyclin C and sestrin 2 in fetal brains. In embryonic neuronal NT2 cells, siRNA repression of separase failed to significantly alter cell turnover, whereas repression of securin expression resulted in increased levels of the activated forms of Rad21 and separase, and promoted cell proliferation. Our data suggest that the co-ordinated expression of separase, securin and Rad21 is fundamental for the developing brain.

U2 - 10.1002/jcp.21086

DO - 10.1002/jcp.21086

M3 - Article

C2 - 17450531

VL - 213

SP - 45

EP - 53

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 1

ER -