Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt

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Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt. / Luechau, F; Ling, TC; Lyddiatt, Andrew.

In: Separation and Purification Technology, Vol. 68, No. 1, 01.06.2009, p. 114-118.

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@article{0f0cd39780454acf931e3e05b2c882ca,
title = "Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt",
abstract = "The selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol (PEG) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPSs) by addition of NaCl salt was studied with pure pDNA and RNA solutions. The pDNA is increasingly excluded from top phases upon the addition of 0.5% and 3% (w/w) NaCl. With 3% (w/w) NaCl, the logarithmic partition coefficient of RNA was 1.2 and as a result, the RNA concentration in the top phase was 3.3-fold higher than that in the bottom phase. It is demonstrated that 47%, 13.7% and 7.5% (w/v) of PEG were required to achieve identical precipitation effects with PEG 300, 1450 and 6000, respectively. The precipitation efficiency of 6.3% (w/v) PEG 300 corresponds to that of 1% (w/v) PEG 6000. The excluded volume effects in the top phase were probably responsible for the selective exclusion of different nucleic acids species. The results obtained in this study contribute to the basic knowledge of partition of macromolecules in ATPSs in terms of excluded volume theory. (C) 2009 Elsevier B.V. All rights reserved.",
keywords = "Selective partition, RNA, Aqueous two-phase systems, Plasmid DNA, NaCl",
author = "F Luechau and TC Ling and Andrew Lyddiatt",
year = "2009",
month = jun,
day = "1",
doi = "10.1016/j.seppur.2009.04.016",
language = "English",
volume = "68",
pages = "114--118",
journal = "Separation and Purification Technology",
issn = "1383-5866",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt

AU - Luechau, F

AU - Ling, TC

AU - Lyddiatt, Andrew

PY - 2009/6/1

Y1 - 2009/6/1

N2 - The selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol (PEG) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPSs) by addition of NaCl salt was studied with pure pDNA and RNA solutions. The pDNA is increasingly excluded from top phases upon the addition of 0.5% and 3% (w/w) NaCl. With 3% (w/w) NaCl, the logarithmic partition coefficient of RNA was 1.2 and as a result, the RNA concentration in the top phase was 3.3-fold higher than that in the bottom phase. It is demonstrated that 47%, 13.7% and 7.5% (w/v) of PEG were required to achieve identical precipitation effects with PEG 300, 1450 and 6000, respectively. The precipitation efficiency of 6.3% (w/v) PEG 300 corresponds to that of 1% (w/v) PEG 6000. The excluded volume effects in the top phase were probably responsible for the selective exclusion of different nucleic acids species. The results obtained in this study contribute to the basic knowledge of partition of macromolecules in ATPSs in terms of excluded volume theory. (C) 2009 Elsevier B.V. All rights reserved.

AB - The selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol (PEG) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPSs) by addition of NaCl salt was studied with pure pDNA and RNA solutions. The pDNA is increasingly excluded from top phases upon the addition of 0.5% and 3% (w/w) NaCl. With 3% (w/w) NaCl, the logarithmic partition coefficient of RNA was 1.2 and as a result, the RNA concentration in the top phase was 3.3-fold higher than that in the bottom phase. It is demonstrated that 47%, 13.7% and 7.5% (w/v) of PEG were required to achieve identical precipitation effects with PEG 300, 1450 and 6000, respectively. The precipitation efficiency of 6.3% (w/v) PEG 300 corresponds to that of 1% (w/v) PEG 6000. The excluded volume effects in the top phase were probably responsible for the selective exclusion of different nucleic acids species. The results obtained in this study contribute to the basic knowledge of partition of macromolecules in ATPSs in terms of excluded volume theory. (C) 2009 Elsevier B.V. All rights reserved.

KW - Selective partition

KW - RNA

KW - Aqueous two-phase systems

KW - Plasmid DNA

KW - NaCl

U2 - 10.1016/j.seppur.2009.04.016

DO - 10.1016/j.seppur.2009.04.016

M3 - Article

VL - 68

SP - 114

EP - 118

JO - Separation and Purification Technology

JF - Separation and Purification Technology

SN - 1383-5866

IS - 1

ER -