Selective partition of plasmid DNA and RNA from crude Escherichia coli cell lysate by aqueous two-phase systems

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

Abstract

In this paper, the partition of plasmid DNA (pDNA) and RNA in polyethylene glycol (PEG) and dipotassium phosphate aqueous two-phase systems (ATPS) by adding up of NaCl salt was studied with crude Escherichia coli (E. coli) cell lysate. The partition of nucleic acid was studied in an ATPS composed of 17% (w/w) PEG300, 14% (w/w) di-potassium phosphate (designated as 17/14) and 40% (w/w) lysate. It is demonstrated that in a 17/14 system with 40% (w/w) lysate, at least 2% (w/w) or 342 mM (kg ATPS)(-1) NaCl were able to selective partition the pDNA to the bottom phase. (C) 2011 Elsevier B.V. All rights reserved.

Details

Original languageEnglish
Pages (from-to)230-232
Number of pages3
JournalBiochemical Engineering Journal
Volume55
Issue number3
Publication statusPublished - 1 Aug 2011

Keywords

  • RNA, Integrated processing, DNA, Purification, Bioseparations, Aqueous two-phase system