Abstract
Mytilus edulis digestive gland microsomes were prepared from indigenous populations sampled from a clean reference site (Port Quin) and an urban-industrial contaminated site (Blackpool) in the UK. Samples were collected in March/April, May, August and December 1998. Western blot analysis was performed using polyclonal antibodies to fish CYP1A and rat CYP2E using partially purified M. edulis CYP as a positive control, to aid identification. CYP1A- and CYP2E-immunopositive protein levels showed different site-specific seasonal variation with higher levels of CYP2E determined in May (P <0.05). At both sites, lower levels of CYP1A-immunopositive protein but not CYP2E-immunopositive protein were observed in the samples collected in December (P <0.05). This correlated with lower levels of nuclear DNA damage (Comet assay expressed as per cent tail DNA) observed in December compared to August (P <0.05).
Original language | English |
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Pages (from-to) | 405-9 |
Number of pages | 5 |
Journal | Marine Environmental Research |
Volume | 50 |
Issue number | 1-5 |
Publication status | Published - 2000 |
Keywords
- Microscopy, Fluorescence
- Bivalvia
- Comet Assay
- Animals
- Digestive System
- Seasons
- Cytochrome P-450 CYP1A1
- Cytochrome P-450 CYP2E1