Schlafen 14 (SLFN14) is a novel antiviral factor involved in the control of viral replication

Rak-Kyun Seong; Seong-Wook Seo; Ji-Ae Kim; Sarah Fletcher; Neil Morgan; Mukesh Kumar; Young-Ki Choi; Ok Sarah Shin

doi:10.1016/j.imbio.2017.07.002

Schlafen 14 (SLFN14) is a novel antiviral factor involved in the control of viral replication

Rak-Kyun Seong, Seong-Wook Seo, Ji-Ae Kim, Sarah Fletcher, Neil Morgan, Mukesh Kumar, Young-Ki Choi, Ok Sarah Shin

Cardiovascular Sciences

Research output: Contribution to journal › Article › peer-review

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Abstract

Schlafen (SLFN) proteins have been suggested to play important functions in cell
proliferation and immune cell development. In this study, we determined the antiviral activities of putative RNA-helicase domain-containing SLFN14. Murine SLFN14 expression was specifically induced by TLR3-mediated pathways and type I interferon (IFN) in RAW264.7 mouse macrophages. To examine the role of SLFN during viral infection, cells were infected with either wild-type PR8 or delNS1/PR8 virus. SLFN14 expression was specifically induced following influenza virus infection. Overexpression of SLFN14 in A549 cells reduced viral replication, whereas knockdown of SLFN14 in RAW264.7 cells enhanced
viral titers. Furthermore, SLFN14 promoted the delay in viral NP translocation from cytoplasm to nucleus and enhanced RIG-I-mediated IFN-signaling. In addition, SLFN14 overexpression promoted antiviral activity against varicella zoster virus (VZV), a DNA virus. In conclusion, our data suggest that SLFN14 is a novel antiviral factor for both DNA and RNA viruses.

Original language	English
Journal	Immunobiology
Early online date	11 Jul 2017
DOIs	https://doi.org/10.1016/j.imbio.2017.07.002
Publication status	E-pub ahead of print - 11 Jul 2017

Keywords

SLFN14
influenza
VZV
interferon
anti-viral

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Seong_et_al_Schalfen_14_(SLFN14)_Immunobiology_2017Accepted author manuscript, 1.48 MBLicence: Creative Commons: Attribution-NonCommercial-NoDerivs (CC BY-NC-ND)

http://www.sciencedirect.com/science/article/pii/S0171298517301158Licence: None: All rights reserved

Cite this

@article{18235ce239e542fe9dc7822981f69c7f,

title = "Schlafen 14 (SLFN14) is a novel antiviral factor involved in the control of viral replication",

abstract = "Schlafen (SLFN) proteins have been suggested to play important functions in cellproliferation and immune cell development. In this study, we determined the antiviral activities of putative RNA-helicase domain-containing SLFN14. Murine SLFN14 expression was specifically induced by TLR3-mediated pathways and type I interferon (IFN) in RAW264.7 mouse macrophages. To examine the role of SLFN during viral infection, cells were infected with either wild-type PR8 or delNS1/PR8 virus. SLFN14 expression was specifically induced following influenza virus infection. Overexpression of SLFN14 in A549 cells reduced viral replication, whereas knockdown of SLFN14 in RAW264.7 cells enhancedviral titers. Furthermore, SLFN14 promoted the delay in viral NP translocation from cytoplasm to nucleus and enhanced RIG-I-mediated IFN-signaling. In addition, SLFN14 overexpression promoted antiviral activity against varicella zoster virus (VZV), a DNA virus. In conclusion, our data suggest that SLFN14 is a novel antiviral factor for both DNA and RNA viruses.",

keywords = "SLFN14 , influenza, VZV , interferon, anti-viral",

author = "Rak-Kyun Seong and Seong-Wook Seo and Ji-Ae Kim and Sarah Fletcher and Neil Morgan and Mukesh Kumar and Young-Ki Choi and Shin, {Ok Sarah}",

year = "2017",

month = jul,

day = "11",

doi = "10.1016/j.imbio.2017.07.002",

language = "English",

journal = "Immunobiology",

issn = "0171-2985",

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TY - JOUR

T1 - Schlafen 14 (SLFN14) is a novel antiviral factor involved in the control of viral replication

AU - Seong, Rak-Kyun

AU - Seo, Seong-Wook

AU - Kim, Ji-Ae

AU - Fletcher, Sarah

AU - Morgan, Neil

AU - Kumar, Mukesh

AU - Choi, Young-Ki

AU - Shin, Ok Sarah

PY - 2017/7/11

Y1 - 2017/7/11

N2 - Schlafen (SLFN) proteins have been suggested to play important functions in cellproliferation and immune cell development. In this study, we determined the antiviral activities of putative RNA-helicase domain-containing SLFN14. Murine SLFN14 expression was specifically induced by TLR3-mediated pathways and type I interferon (IFN) in RAW264.7 mouse macrophages. To examine the role of SLFN during viral infection, cells were infected with either wild-type PR8 or delNS1/PR8 virus. SLFN14 expression was specifically induced following influenza virus infection. Overexpression of SLFN14 in A549 cells reduced viral replication, whereas knockdown of SLFN14 in RAW264.7 cells enhancedviral titers. Furthermore, SLFN14 promoted the delay in viral NP translocation from cytoplasm to nucleus and enhanced RIG-I-mediated IFN-signaling. In addition, SLFN14 overexpression promoted antiviral activity against varicella zoster virus (VZV), a DNA virus. In conclusion, our data suggest that SLFN14 is a novel antiviral factor for both DNA and RNA viruses.

AB - Schlafen (SLFN) proteins have been suggested to play important functions in cellproliferation and immune cell development. In this study, we determined the antiviral activities of putative RNA-helicase domain-containing SLFN14. Murine SLFN14 expression was specifically induced by TLR3-mediated pathways and type I interferon (IFN) in RAW264.7 mouse macrophages. To examine the role of SLFN during viral infection, cells were infected with either wild-type PR8 or delNS1/PR8 virus. SLFN14 expression was specifically induced following influenza virus infection. Overexpression of SLFN14 in A549 cells reduced viral replication, whereas knockdown of SLFN14 in RAW264.7 cells enhancedviral titers. Furthermore, SLFN14 promoted the delay in viral NP translocation from cytoplasm to nucleus and enhanced RIG-I-mediated IFN-signaling. In addition, SLFN14 overexpression promoted antiviral activity against varicella zoster virus (VZV), a DNA virus. In conclusion, our data suggest that SLFN14 is a novel antiviral factor for both DNA and RNA viruses.

KW - SLFN14

KW - influenza

KW - VZV

KW - interferon

KW - anti-viral

U2 - 10.1016/j.imbio.2017.07.002

DO - 10.1016/j.imbio.2017.07.002

M3 - Article

SN - 0171-2985

JO - Immunobiology

JF - Immunobiology

ER -

Schlafen 14 (SLFN14) is a novel antiviral factor involved in the control of viral replication

Abstract

Keywords

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