Scan, extract, wrap, compute: a 3D method to analyse morphological shape differences

Research output: Contribution to journalArticlepeer-review

Authors

  • Martin Horstmann
  • Petra Stamm
  • Sebastian Kruppert
  • Ralph Tollrian
  • Linda C. Weiss

Colleges, School and Institutes

External organisations

  • Universitat Bochum

Abstract

Quantitative analysis of shape and form is critical in many biological disciplines, as context-dependent morphotypes reflect changes in gene expression and physiology, e.g., in comparisons of environment-dependent phenotypes, forward/reverse genetic assays or shape development during ontogenesis. 3D-shape rendering methods produce models with arbitrarily numbered, and therefore non-comparable, mesh points. However, this prevents direct comparisons. We introduce a workflow that allows the generation of comparable 3D models based on several specimens. Translocations between points of modelled morphotypes are plotted as heat maps and statistically tested. With this workflow, we are able to detect, model and investigate the significance of shape and form alterations in all spatial dimensions, demonstrated with different morphotypes of the pond-dwelling microcrustacean Daphnia. Furthermore, it allows the detection even of inconspicuous morphological features that can be exported to programs for subsequent analysis, e.g., streamline- or finite-element analysis.

Details

Original languageEnglish
Article numbere4861
Number of pages20
JournalPeerJ
Volume2018
Issue number6
Publication statusPublished - 8 Jun 2018

Keywords

  • 3D morphological comparison, 3D morphology, Confidence ellipsoids, Confocal microscopy, Daphnia, Landmark-rare shapes, Shape and form analysis, Visualisation of shape alteration