TY - JOUR
T1 - Scalable synthesis of multicolour conjugated polymer nanoparticles via Suzuki-Miyaura polymerisation in a miniemulsion and application in bioimaging
AU - Behrendt, Jonathan M.
AU - Guzman, Jair A. Esquivel
AU - Purdie, Laura
AU - Willcock, Helen
AU - Morrison, John J.
AU - Foster, Andrew B.
AU - O'Reilly, Rachel K.
AU - McCairn, Mark C.
AU - Turner, Michael L.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Suzuki cross-coupling polymerisation of aryldibromides and aryldiboronate esters in a sodium dodecyl sulfate (SDS)-stabilised miniemulsion provides a versatile and direct route to fluorescent conjugated polymer nanoparticles (CPNs). These nanoparticles have a conjugated backbone based on poly(9,9-dioctylfluorene) (PFO), however, significant structural diversity is introduced by incorporation of electron withdrawing, heterocyclic comonomers (5–50 mol.%) in order to tune the emission wavelengths from blue to far-red/near-infrared. The robust nature of the polymerisation methodology allows for rapid assessment of the relationship between polymer composition, chain morphology and optical properties of the resultant CPNs. Moreover, the CPNs (after a simple and rapid purification step) can be used directly in fluorescence-based intracellular labelling experiments (in HCT116 cells), in which they display low cytotoxicity at biologically-useful concentrations.
AB - Suzuki cross-coupling polymerisation of aryldibromides and aryldiboronate esters in a sodium dodecyl sulfate (SDS)-stabilised miniemulsion provides a versatile and direct route to fluorescent conjugated polymer nanoparticles (CPNs). These nanoparticles have a conjugated backbone based on poly(9,9-dioctylfluorene) (PFO), however, significant structural diversity is introduced by incorporation of electron withdrawing, heterocyclic comonomers (5–50 mol.%) in order to tune the emission wavelengths from blue to far-red/near-infrared. The robust nature of the polymerisation methodology allows for rapid assessment of the relationship between polymer composition, chain morphology and optical properties of the resultant CPNs. Moreover, the CPNs (after a simple and rapid purification step) can be used directly in fluorescence-based intracellular labelling experiments (in HCT116 cells), in which they display low cytotoxicity at biologically-useful concentrations.
KW - conjugated polymer nanoparticles
KW - miniemulsion
KW - Suzuki-Miyaura polymerisation
KW - scaleable
KW - bioimaging
U2 - 10.1016/j.reactfunctpolym.2016.08.006
DO - 10.1016/j.reactfunctpolym.2016.08.006
M3 - Article
SN - 1381-5148
VL - 107
SP - 69
EP - 77
JO - Reactive and Functional Polymers
JF - Reactive and Functional Polymers
ER -