Salmonella enterica serovar Typhimurium travels to mesenteric lymph nodes both with host cells and autonomously

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Salmonella enterica serovar Typhimurium travels to mesenteric lymph nodes both with host cells and autonomously. / Bravo-Blas, Alberto; Utriainen, Lotta; Clay, Slater L; Kästele, Verena; Cerovic, Vuk; Cunningham, Adam F; Henderson, Ian R; Wall, Daniel M; Milling, Simon W F.

In: Journal of Immunology, Vol. 202, No. 1, 01.01.2019, p. 260-267.

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Bravo-Blas, Alberto ; Utriainen, Lotta ; Clay, Slater L ; Kästele, Verena ; Cerovic, Vuk ; Cunningham, Adam F ; Henderson, Ian R ; Wall, Daniel M ; Milling, Simon W F. / Salmonella enterica serovar Typhimurium travels to mesenteric lymph nodes both with host cells and autonomously. In: Journal of Immunology. 2019 ; Vol. 202, No. 1. pp. 260-267.

Bibtex

@article{117d1a6037f04322bfd2334130e13a61,
title = "Salmonella enterica serovar Typhimurium travels to mesenteric lymph nodes both with host cells and autonomously",
abstract = "Salmonella infection is a globally important cause of gastroenteritis and systemic disease and is a useful tool to study immune responses in the intestine. Although mechanisms leading to immune responses against Salmonella have been extensively studied, questions remain about how bacteria travel from the intestinal mucosa to the mesenteric lymph nodes (MLN), a key site for Ag presentation. In this study, we used a mouse model of infection with Salmonella enterica serovar Typhimurium (STM) to identify changes in intestinal immune cells induced during early infection. We then used fluorescently labeled STM to identify interactions with immune cells from the site of infection through migration in lymph to the MLN. We show that viable STM can be carried in the lymph by any subset of migrating dendritic cells but not by macrophages. Moreover, approximately half of the STM in lymph are not associated with cells at all and travel autonomously. Within the MLN, STM associates with dendritic cells and B cells but predominantly with MLN-resident macrophages. In conclusion, we describe the routes used by STM to spread systemically in the period immediately postinfection. This deeper understanding of the infection process could open new avenues for controlling it.",
author = "Alberto Bravo-Blas and Lotta Utriainen and Clay, {Slater L} and Verena K{\"a}stele and Vuk Cerovic and Cunningham, {Adam F} and Henderson, {Ian R} and Wall, {Daniel M} and Milling, {Simon W F}",
note = "Copyright {\textcopyright} 2018 The Authors.",
year = "2019",
month = jan
day = "1",
doi = "10.4049/jimmunol.1701254",
language = "English",
volume = "202",
pages = "260--267",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "1",

}

RIS

TY - JOUR

T1 - Salmonella enterica serovar Typhimurium travels to mesenteric lymph nodes both with host cells and autonomously

AU - Bravo-Blas, Alberto

AU - Utriainen, Lotta

AU - Clay, Slater L

AU - Kästele, Verena

AU - Cerovic, Vuk

AU - Cunningham, Adam F

AU - Henderson, Ian R

AU - Wall, Daniel M

AU - Milling, Simon W F

N1 - Copyright © 2018 The Authors.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Salmonella infection is a globally important cause of gastroenteritis and systemic disease and is a useful tool to study immune responses in the intestine. Although mechanisms leading to immune responses against Salmonella have been extensively studied, questions remain about how bacteria travel from the intestinal mucosa to the mesenteric lymph nodes (MLN), a key site for Ag presentation. In this study, we used a mouse model of infection with Salmonella enterica serovar Typhimurium (STM) to identify changes in intestinal immune cells induced during early infection. We then used fluorescently labeled STM to identify interactions with immune cells from the site of infection through migration in lymph to the MLN. We show that viable STM can be carried in the lymph by any subset of migrating dendritic cells but not by macrophages. Moreover, approximately half of the STM in lymph are not associated with cells at all and travel autonomously. Within the MLN, STM associates with dendritic cells and B cells but predominantly with MLN-resident macrophages. In conclusion, we describe the routes used by STM to spread systemically in the period immediately postinfection. This deeper understanding of the infection process could open new avenues for controlling it.

AB - Salmonella infection is a globally important cause of gastroenteritis and systemic disease and is a useful tool to study immune responses in the intestine. Although mechanisms leading to immune responses against Salmonella have been extensively studied, questions remain about how bacteria travel from the intestinal mucosa to the mesenteric lymph nodes (MLN), a key site for Ag presentation. In this study, we used a mouse model of infection with Salmonella enterica serovar Typhimurium (STM) to identify changes in intestinal immune cells induced during early infection. We then used fluorescently labeled STM to identify interactions with immune cells from the site of infection through migration in lymph to the MLN. We show that viable STM can be carried in the lymph by any subset of migrating dendritic cells but not by macrophages. Moreover, approximately half of the STM in lymph are not associated with cells at all and travel autonomously. Within the MLN, STM associates with dendritic cells and B cells but predominantly with MLN-resident macrophages. In conclusion, we describe the routes used by STM to spread systemically in the period immediately postinfection. This deeper understanding of the infection process could open new avenues for controlling it.

U2 - 10.4049/jimmunol.1701254

DO - 10.4049/jimmunol.1701254

M3 - Article

C2 - 30487173

VL - 202

SP - 260

EP - 267

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 1

ER -