Revealing the nanostructure of glyceryl tristearate crystals by atomic force microscopy

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Revealing the nanostructure of glyceryl tristearate crystals by atomic force microscopy. / Gao, Nan; Sebben, Damien; Gillies, Graeme; Krasowska, Marta; Beattie, David.

In: Crystal Growth and Design, Vol. 19, No. 1, 02.01.2019, p. 513-519.

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Gao, Nan ; Sebben, Damien ; Gillies, Graeme ; Krasowska, Marta ; Beattie, David. / Revealing the nanostructure of glyceryl tristearate crystals by atomic force microscopy. In: Crystal Growth and Design. 2019 ; Vol. 19, No. 1. pp. 513-519.

Bibtex

@article{83b23e0c6fd9441fa58115d7d7cec86a,
title = "Revealing the nanostructure of glyceryl tristearate crystals by atomic force microscopy",
abstract = "Habit and morphology of triglyceride crystals are critical factors in the formulation of lipid-based colloidal systems such as pharmaceutical formulations and dairy products. Until now, triglyceride crystal characterization has been largely performed using thermal analysis, scattering techniques, and electron microscopy. We present the use of Atomic Force Microscopy (AFM) for the 3-dimensional mapping of the nanoscale morphology of single glyceryl tristearate (tristearin) crystals. We show in great detail the topographical features of tristearin crystals not apparent using other techniques, and investigate the effect of solvent-mediated recrystallization on crystal morphology. Specifically, recrystallization from toluene results in the production of well-ordered crystals with a regular lamellar thickness of 5 nm, and a habit that depends on the rate of evaporation. X-ray diffraction (XRD) analysis confirms that the crystalline phase prior to and following recrystallization is maintained; however, the bulky lamellar structure is replaced with a thin, ordered structure. The use of AFM circumvents the disadvantages of electron microscopy, such as melting of triglyceride crystals and freezing of aqueous phase residue. The approach presented in this work gives new physical insight into the 3-dimensional features of the crystals, which will have an influence of their behavior in lipid-based soft matter and colloidal systems.",
author = "Nan Gao and Damien Sebben and Graeme Gillies and Marta Krasowska and David Beattie",
year = "2019",
month = jan,
day = "2",
doi = "10.1021/acs.cgd.8b01699",
language = "English",
volume = "19",
pages = "513--519",
journal = "Crystal Growth and Design",
issn = "1528-7483",
publisher = "American Chemical Society",
number = "1",

}

RIS

TY - JOUR

T1 - Revealing the nanostructure of glyceryl tristearate crystals by atomic force microscopy

AU - Gao, Nan

AU - Sebben, Damien

AU - Gillies, Graeme

AU - Krasowska, Marta

AU - Beattie, David

PY - 2019/1/2

Y1 - 2019/1/2

N2 - Habit and morphology of triglyceride crystals are critical factors in the formulation of lipid-based colloidal systems such as pharmaceutical formulations and dairy products. Until now, triglyceride crystal characterization has been largely performed using thermal analysis, scattering techniques, and electron microscopy. We present the use of Atomic Force Microscopy (AFM) for the 3-dimensional mapping of the nanoscale morphology of single glyceryl tristearate (tristearin) crystals. We show in great detail the topographical features of tristearin crystals not apparent using other techniques, and investigate the effect of solvent-mediated recrystallization on crystal morphology. Specifically, recrystallization from toluene results in the production of well-ordered crystals with a regular lamellar thickness of 5 nm, and a habit that depends on the rate of evaporation. X-ray diffraction (XRD) analysis confirms that the crystalline phase prior to and following recrystallization is maintained; however, the bulky lamellar structure is replaced with a thin, ordered structure. The use of AFM circumvents the disadvantages of electron microscopy, such as melting of triglyceride crystals and freezing of aqueous phase residue. The approach presented in this work gives new physical insight into the 3-dimensional features of the crystals, which will have an influence of their behavior in lipid-based soft matter and colloidal systems.

AB - Habit and morphology of triglyceride crystals are critical factors in the formulation of lipid-based colloidal systems such as pharmaceutical formulations and dairy products. Until now, triglyceride crystal characterization has been largely performed using thermal analysis, scattering techniques, and electron microscopy. We present the use of Atomic Force Microscopy (AFM) for the 3-dimensional mapping of the nanoscale morphology of single glyceryl tristearate (tristearin) crystals. We show in great detail the topographical features of tristearin crystals not apparent using other techniques, and investigate the effect of solvent-mediated recrystallization on crystal morphology. Specifically, recrystallization from toluene results in the production of well-ordered crystals with a regular lamellar thickness of 5 nm, and a habit that depends on the rate of evaporation. X-ray diffraction (XRD) analysis confirms that the crystalline phase prior to and following recrystallization is maintained; however, the bulky lamellar structure is replaced with a thin, ordered structure. The use of AFM circumvents the disadvantages of electron microscopy, such as melting of triglyceride crystals and freezing of aqueous phase residue. The approach presented in this work gives new physical insight into the 3-dimensional features of the crystals, which will have an influence of their behavior in lipid-based soft matter and colloidal systems.

U2 - 10.1021/acs.cgd.8b01699

DO - 10.1021/acs.cgd.8b01699

M3 - Article

VL - 19

SP - 513

EP - 519

JO - Crystal Growth and Design

JF - Crystal Growth and Design

SN - 1528-7483

IS - 1

ER -