Response heterogeneity of human macrophages to ATP is associated with P2X7 receptor expression but not to polymorphisms in the P2RX7 promoter

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@article{8e162f9fa50f4c2b9d4ebf92e1a2c3ec,
title = "Response heterogeneity of human macrophages to ATP is associated with P2X7 receptor expression but not to polymorphisms in the P2RX7 promoter",
abstract = "A region 2 kb upstream of exon 1 of the P2X7 gene was sequenced using DNA from nine healthy individuals who exhibited three different ATP response phenotypes (i.e. high, low and interferon gamma-inducible). Five single nucleotide polymorphisms were identified within the nine donor promoter sequences but none were associated with a specific ATP response phenotype. A P2X7 loss of function polymorphism (1513 in exon 13) was also screened for within donor DNA but no response associations were identified. ATP response phenotype was positively associated with P2X(7) receptor expression, as assessed by flow cytometry, but not with any identified receptor or promoter gene polymorphisms.",
keywords = "promoter, 1513, P2X(7), polymorphism, ATP, cell lysis",
author = "Cheuk Li and S Campbell and Dinakantha Kumararatne and AVS Hill and David Lammas",
year = "2002",
month = nov,
day = "6",
doi = "10.1016/S0014-5793(02)03424-5",
language = "English",
volume = "531",
pages = "127--131",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Response heterogeneity of human macrophages to ATP is associated with P2X7 receptor expression but not to polymorphisms in the P2RX7 promoter

AU - Li, Cheuk

AU - Campbell, S

AU - Kumararatne, Dinakantha

AU - Hill, AVS

AU - Lammas, David

PY - 2002/11/6

Y1 - 2002/11/6

N2 - A region 2 kb upstream of exon 1 of the P2X7 gene was sequenced using DNA from nine healthy individuals who exhibited three different ATP response phenotypes (i.e. high, low and interferon gamma-inducible). Five single nucleotide polymorphisms were identified within the nine donor promoter sequences but none were associated with a specific ATP response phenotype. A P2X7 loss of function polymorphism (1513 in exon 13) was also screened for within donor DNA but no response associations were identified. ATP response phenotype was positively associated with P2X(7) receptor expression, as assessed by flow cytometry, but not with any identified receptor or promoter gene polymorphisms.

AB - A region 2 kb upstream of exon 1 of the P2X7 gene was sequenced using DNA from nine healthy individuals who exhibited three different ATP response phenotypes (i.e. high, low and interferon gamma-inducible). Five single nucleotide polymorphisms were identified within the nine donor promoter sequences but none were associated with a specific ATP response phenotype. A P2X7 loss of function polymorphism (1513 in exon 13) was also screened for within donor DNA but no response associations were identified. ATP response phenotype was positively associated with P2X(7) receptor expression, as assessed by flow cytometry, but not with any identified receptor or promoter gene polymorphisms.

KW - promoter

KW - 1513

KW - P2X(7)

KW - polymorphism

KW - ATP

KW - cell lysis

UR - http://www.scopus.com/inward/record.url?scp=0037032444&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(02)03424-5

DO - 10.1016/S0014-5793(02)03424-5

M3 - Article

C2 - 12417299

VL - 531

SP - 127

EP - 131

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -