Resistance exercise enhances myofibrillar protein synthesis with graded intakes of whey protein in older men

Research output: Contribution to journalArticlepeer-review

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Resistance exercise enhances myofibrillar protein synthesis with graded intakes of whey protein in older men. / Yang, Yifan; Breen, Leigh; Burd, Nicholas A; Hector, Amy J; Churchward-Venne, Tyler A; Josse, Andrea R; Tarnopolsky, M A; Phillips, Stuart M.

In: The British journal of nutrition, Vol. 108, No. 10, 28.11.2012, p. 1780-8.

Research output: Contribution to journalArticlepeer-review

Harvard

Yang, Y, Breen, L, Burd, NA, Hector, AJ, Churchward-Venne, TA, Josse, AR, Tarnopolsky, MA & Phillips, SM 2012, 'Resistance exercise enhances myofibrillar protein synthesis with graded intakes of whey protein in older men', The British journal of nutrition, vol. 108, no. 10, pp. 1780-8. https://doi.org/10.1017/S0007114511007422

APA

Yang, Y., Breen, L., Burd, N. A., Hector, A. J., Churchward-Venne, T. A., Josse, A. R., Tarnopolsky, M. A., & Phillips, S. M. (2012). Resistance exercise enhances myofibrillar protein synthesis with graded intakes of whey protein in older men. The British journal of nutrition, 108(10), 1780-8. https://doi.org/10.1017/S0007114511007422

Vancouver

Author

Yang, Yifan ; Breen, Leigh ; Burd, Nicholas A ; Hector, Amy J ; Churchward-Venne, Tyler A ; Josse, Andrea R ; Tarnopolsky, M A ; Phillips, Stuart M. / Resistance exercise enhances myofibrillar protein synthesis with graded intakes of whey protein in older men. In: The British journal of nutrition. 2012 ; Vol. 108, No. 10. pp. 1780-8.

Bibtex

@article{c5cce6840f1148d580f2e824afd04abd,
title = "Resistance exercise enhances myofibrillar protein synthesis with graded intakes of whey protein in older men",
abstract = "Feeding stimulates robust increases in muscle protein synthesis (MPS); however, ageing may alter the anabolic response to protein ingestion and the subsequent aminoacidaemia. With this as background, we aimed to determine in the present study the dose-response of MPS with the ingestion of isolated whey protein, with and without prior resistance exercise, in the elderly. For the purpose of this study, thirty-seven elderly men (age 71 (sd 4) years) completed a bout of unilateral leg-based resistance exercise before ingesting 0, 10, 20 or 40 g of whey protein isolate (W0-W40, respectively). Infusion of l-[1-13C]leucine and l-[ring-13C6]phenylalanine with bilateral vastus lateralis muscle biopsies were used to ascertain whole-body leucine oxidation and 4 h post-protein consumption of MPS in the fed-state of non-exercised and exercised leg muscles. It was determined that whole-body leucine oxidation increased in a stepwise, dose-dependent manner. MPS increased above basal, fasting values by approximately 65 and 90 % for W20 and W40, respectively (P <0·05), but not with lower doses of whey. While resistance exercise was generally effective at stimulating MPS, W20 and W40 ingestion post-exercise increased MPS above W0 and W10 exercised values (P <0·05) and W40 was greater than W20 (P <0·05). Based on the study, the following conclusions were drawn. At rest, the optimal whey protein dose for non-frail older adults to consume, to increase myofibrillar MPS above fasting rates, was 20 g. Resistance exercise increases MPS in the elderly at all protein doses, but to a greater extent with 40 g of whey ingestion. These data suggest that, in contrast to younger adults, in whom post-exercise rates of MPS are saturated with 20 g of protein, exercised muscles of older adults respond to higher protein doses.",
keywords = "Amino Acids, Food Analysis, Humans, Aged, Exercise, Insulin, Myofibrils, Dietary Supplements, Gene Expression Regulation, Milk Proteins, Diet, Male, Carbon Isotopes",
author = "Yifan Yang and Leigh Breen and Burd, {Nicholas A} and Hector, {Amy J} and Churchward-Venne, {Tyler A} and Josse, {Andrea R} and Tarnopolsky, {M A} and Phillips, {Stuart M}",
year = "2012",
month = nov,
day = "28",
doi = "10.1017/S0007114511007422",
language = "English",
volume = "108",
pages = "1780--8",
journal = "The British journal of nutrition",
issn = "0007-1145",
publisher = "Cambridge University Press",
number = "10",

}

RIS

TY - JOUR

T1 - Resistance exercise enhances myofibrillar protein synthesis with graded intakes of whey protein in older men

AU - Yang, Yifan

AU - Breen, Leigh

AU - Burd, Nicholas A

AU - Hector, Amy J

AU - Churchward-Venne, Tyler A

AU - Josse, Andrea R

AU - Tarnopolsky, M A

AU - Phillips, Stuart M

PY - 2012/11/28

Y1 - 2012/11/28

N2 - Feeding stimulates robust increases in muscle protein synthesis (MPS); however, ageing may alter the anabolic response to protein ingestion and the subsequent aminoacidaemia. With this as background, we aimed to determine in the present study the dose-response of MPS with the ingestion of isolated whey protein, with and without prior resistance exercise, in the elderly. For the purpose of this study, thirty-seven elderly men (age 71 (sd 4) years) completed a bout of unilateral leg-based resistance exercise before ingesting 0, 10, 20 or 40 g of whey protein isolate (W0-W40, respectively). Infusion of l-[1-13C]leucine and l-[ring-13C6]phenylalanine with bilateral vastus lateralis muscle biopsies were used to ascertain whole-body leucine oxidation and 4 h post-protein consumption of MPS in the fed-state of non-exercised and exercised leg muscles. It was determined that whole-body leucine oxidation increased in a stepwise, dose-dependent manner. MPS increased above basal, fasting values by approximately 65 and 90 % for W20 and W40, respectively (P <0·05), but not with lower doses of whey. While resistance exercise was generally effective at stimulating MPS, W20 and W40 ingestion post-exercise increased MPS above W0 and W10 exercised values (P <0·05) and W40 was greater than W20 (P <0·05). Based on the study, the following conclusions were drawn. At rest, the optimal whey protein dose for non-frail older adults to consume, to increase myofibrillar MPS above fasting rates, was 20 g. Resistance exercise increases MPS in the elderly at all protein doses, but to a greater extent with 40 g of whey ingestion. These data suggest that, in contrast to younger adults, in whom post-exercise rates of MPS are saturated with 20 g of protein, exercised muscles of older adults respond to higher protein doses.

AB - Feeding stimulates robust increases in muscle protein synthesis (MPS); however, ageing may alter the anabolic response to protein ingestion and the subsequent aminoacidaemia. With this as background, we aimed to determine in the present study the dose-response of MPS with the ingestion of isolated whey protein, with and without prior resistance exercise, in the elderly. For the purpose of this study, thirty-seven elderly men (age 71 (sd 4) years) completed a bout of unilateral leg-based resistance exercise before ingesting 0, 10, 20 or 40 g of whey protein isolate (W0-W40, respectively). Infusion of l-[1-13C]leucine and l-[ring-13C6]phenylalanine with bilateral vastus lateralis muscle biopsies were used to ascertain whole-body leucine oxidation and 4 h post-protein consumption of MPS in the fed-state of non-exercised and exercised leg muscles. It was determined that whole-body leucine oxidation increased in a stepwise, dose-dependent manner. MPS increased above basal, fasting values by approximately 65 and 90 % for W20 and W40, respectively (P <0·05), but not with lower doses of whey. While resistance exercise was generally effective at stimulating MPS, W20 and W40 ingestion post-exercise increased MPS above W0 and W10 exercised values (P <0·05) and W40 was greater than W20 (P <0·05). Based on the study, the following conclusions were drawn. At rest, the optimal whey protein dose for non-frail older adults to consume, to increase myofibrillar MPS above fasting rates, was 20 g. Resistance exercise increases MPS in the elderly at all protein doses, but to a greater extent with 40 g of whey ingestion. These data suggest that, in contrast to younger adults, in whom post-exercise rates of MPS are saturated with 20 g of protein, exercised muscles of older adults respond to higher protein doses.

KW - Amino Acids

KW - Food Analysis

KW - Humans

KW - Aged

KW - Exercise

KW - Insulin

KW - Myofibrils

KW - Dietary Supplements

KW - Gene Expression Regulation

KW - Milk Proteins

KW - Diet

KW - Male

KW - Carbon Isotopes

U2 - 10.1017/S0007114511007422

DO - 10.1017/S0007114511007422

M3 - Article

C2 - 22313809

VL - 108

SP - 1780

EP - 1788

JO - The British journal of nutrition

JF - The British journal of nutrition

SN - 0007-1145

IS - 10

ER -