Remasking of Candida albicans β-Glucan in response to environmental pH is regulated by quorum sensing

Research output: Contribution to journalArticlepeer-review

Standard

Remasking of Candida albicans β-Glucan in response to environmental pH is regulated by quorum sensing. / Cottier, Fabien; Sherrington, Sarah; Cockerill, Sarah; del Olmo Toledo, Valentina; Kissane, Stephen; Tournu, Helene; Orsini, Luisa; Palmer, Glen; Perez, J. Christian; Hall, Rebecca.

In: mBio, Vol. 10, No. 5, e02347-19, 15.10.2019.

Research output: Contribution to journalArticlepeer-review

Harvard

Cottier, F, Sherrington, S, Cockerill, S, del Olmo Toledo, V, Kissane, S, Tournu, H, Orsini, L, Palmer, G, Perez, JC & Hall, R 2019, 'Remasking of Candida albicans β-Glucan in response to environmental pH is regulated by quorum sensing', mBio, vol. 10, no. 5, e02347-19. https://doi.org/10.1128/mBio.02347-19

APA

Cottier, F., Sherrington, S., Cockerill, S., del Olmo Toledo, V., Kissane, S., Tournu, H., Orsini, L., Palmer, G., Perez, J. C., & Hall, R. (2019). Remasking of Candida albicans β-Glucan in response to environmental pH is regulated by quorum sensing. mBio, 10(5), [e02347-19]. https://doi.org/10.1128/mBio.02347-19

Vancouver

Cottier F, Sherrington S, Cockerill S, del Olmo Toledo V, Kissane S, Tournu H et al. Remasking of Candida albicans β-Glucan in response to environmental pH is regulated by quorum sensing. mBio. 2019 Oct 15;10(5). e02347-19. https://doi.org/10.1128/mBio.02347-19

Author

Cottier, Fabien ; Sherrington, Sarah ; Cockerill, Sarah ; del Olmo Toledo, Valentina ; Kissane, Stephen ; Tournu, Helene ; Orsini, Luisa ; Palmer, Glen ; Perez, J. Christian ; Hall, Rebecca. / Remasking of Candida albicans β-Glucan in response to environmental pH is regulated by quorum sensing. In: mBio. 2019 ; Vol. 10, No. 5.

Bibtex

@article{da6c241e16fa497ea6c8ca33ab0f168a,
title = "Remasking of Candida albicans β-Glucan in response to environmental pH is regulated by quorum sensing",
abstract = "Candida albicans is a commensal yeast of the human gut which is tolerated by the immune system but has the potential to become an opportunistic pathogen. One way in which C. albicans achieves this duality is through concealing or exposing cell wall pathogen-associated molecular patterns (PAMPs) in response to hostderived environment cues (pH, hypoxia, and lactate). This cell wall remodeling allows C. albicans to evade or hyperactivate the host{\textquoteright}s innate immune responses, leading to disease. Previously, we showed that adaptation of C. albicans to acidic environments, conditions encountered during colonization of the female reproductive tract, induces significant cell wall remodeling resulting in the exposure of two key fungal PAMPs (-glucan and chitin). Here, we report that this pH-dependent cell wall remodeling is time dependent, with the initial change in pH driving cell wall unmasking, which is then remasked at later time points. Remasking of -glucan was mediated via the cell density-dependent fungal quorum sensing molecule farnesol, while chitin remasking was mediated via a small, heat-stable, nonproteinaceous secreted molecule(s). Transcript profiling identified a core set of 42 genes significantly regulated by pH over time and identified the transcription factor Efg1 as a regulator of chitin exposure through regulation of CHT2. This dynamic cell wall remodeling influenced innate immune recognition of C. albicans, suggesting that during infection, C. albicans can manipulate the host innate immune responses.",
keywords = "Candida albicans, cell wall, chitin, glucans, pH sensing, quorum sensing",
author = "Fabien Cottier and Sarah Sherrington and Sarah Cockerill and {del Olmo Toledo}, Valentina and Stephen Kissane and Helene Tournu and Luisa Orsini and Glen Palmer and Perez, {J. Christian} and Rebecca Hall",
year = "2019",
month = oct,
day = "15",
doi = "10.1128/mBio.02347-19",
language = "English",
volume = "10",
journal = "mBio",
issn = "2150-7511",
publisher = "American Society for Microbiology",
number = "5",

}

RIS

TY - JOUR

T1 - Remasking of Candida albicans β-Glucan in response to environmental pH is regulated by quorum sensing

AU - Cottier, Fabien

AU - Sherrington, Sarah

AU - Cockerill, Sarah

AU - del Olmo Toledo, Valentina

AU - Kissane, Stephen

AU - Tournu, Helene

AU - Orsini, Luisa

AU - Palmer, Glen

AU - Perez, J. Christian

AU - Hall, Rebecca

PY - 2019/10/15

Y1 - 2019/10/15

N2 - Candida albicans is a commensal yeast of the human gut which is tolerated by the immune system but has the potential to become an opportunistic pathogen. One way in which C. albicans achieves this duality is through concealing or exposing cell wall pathogen-associated molecular patterns (PAMPs) in response to hostderived environment cues (pH, hypoxia, and lactate). This cell wall remodeling allows C. albicans to evade or hyperactivate the host’s innate immune responses, leading to disease. Previously, we showed that adaptation of C. albicans to acidic environments, conditions encountered during colonization of the female reproductive tract, induces significant cell wall remodeling resulting in the exposure of two key fungal PAMPs (-glucan and chitin). Here, we report that this pH-dependent cell wall remodeling is time dependent, with the initial change in pH driving cell wall unmasking, which is then remasked at later time points. Remasking of -glucan was mediated via the cell density-dependent fungal quorum sensing molecule farnesol, while chitin remasking was mediated via a small, heat-stable, nonproteinaceous secreted molecule(s). Transcript profiling identified a core set of 42 genes significantly regulated by pH over time and identified the transcription factor Efg1 as a regulator of chitin exposure through regulation of CHT2. This dynamic cell wall remodeling influenced innate immune recognition of C. albicans, suggesting that during infection, C. albicans can manipulate the host innate immune responses.

AB - Candida albicans is a commensal yeast of the human gut which is tolerated by the immune system but has the potential to become an opportunistic pathogen. One way in which C. albicans achieves this duality is through concealing or exposing cell wall pathogen-associated molecular patterns (PAMPs) in response to hostderived environment cues (pH, hypoxia, and lactate). This cell wall remodeling allows C. albicans to evade or hyperactivate the host’s innate immune responses, leading to disease. Previously, we showed that adaptation of C. albicans to acidic environments, conditions encountered during colonization of the female reproductive tract, induces significant cell wall remodeling resulting in the exposure of two key fungal PAMPs (-glucan and chitin). Here, we report that this pH-dependent cell wall remodeling is time dependent, with the initial change in pH driving cell wall unmasking, which is then remasked at later time points. Remasking of -glucan was mediated via the cell density-dependent fungal quorum sensing molecule farnesol, while chitin remasking was mediated via a small, heat-stable, nonproteinaceous secreted molecule(s). Transcript profiling identified a core set of 42 genes significantly regulated by pH over time and identified the transcription factor Efg1 as a regulator of chitin exposure through regulation of CHT2. This dynamic cell wall remodeling influenced innate immune recognition of C. albicans, suggesting that during infection, C. albicans can manipulate the host innate immune responses.

KW - Candida albicans

KW - cell wall

KW - chitin

KW - glucans

KW - pH sensing

KW - quorum sensing

UR - http://www.scopus.com/inward/record.url?scp=85073479680&partnerID=8YFLogxK

U2 - 10.1128/mBio.02347-19

DO - 10.1128/mBio.02347-19

M3 - Article

C2 - 31615961

VL - 10

JO - mBio

JF - mBio

SN - 2150-7511

IS - 5

M1 - e02347-19

ER -