Regulation of Chicken Lysozyme Gene Expression in Transgenic Mice

Research output: Contribution to journalArticle

Standard

Regulation of Chicken Lysozyme Gene Expression in Transgenic Mice. / Bonifer, Constanze; Huber, M; Faust, N; Sippel, AE.

In: Critical Reviews in Eukaryotic Gene Expression, Vol. 6, No. 2-3, 1996, p. 285-297.

Research output: Contribution to journalArticle

Harvard

APA

Vancouver

Author

Bibtex

@article{abbeee8fe91d4513ac5022292da87f99,
title = "Regulation of Chicken Lysozyme Gene Expression in Transgenic Mice",
abstract = "The chicken lysozyme locus is transcriptionally activated during macrophage differentiation. Each cis-regulatory element has its unique activation stage during cell differentiation, whereby maximal transcriptional activity of the gene is only observed when all cis-elements are active. The complete chicken lysozyme locus is expressed position independently and at a high level in macrophages of transgenic mice. For correct transgene regulation, the cooperation of all cis-regulatory elements is required. These cis-regulatory elements specify the mode of regulation and we observe the same expression pattern of the transgene in the mouse and the endogenous gene in chicken macrophages. This indicates that the transcription factors responsible for chicken lysozyme regulation are highly conserved in evolution. The endogenous mouse lysozyme gene is regulated differently. The chromatin of the lysozyme locus is highly structured in the transcriptionally active, as well as in the inactive state. The transcriptional activation of the lysozyme locus is accompanied by extensive chromatin rearrangements, which are disturbed when one essential cis-regulatory element is deleted and the transgenes are subjects to genomic position effects. Based on these results, we propose that a distinct chromatin architecture of a gene locus is required for its correct activation.",
keywords = "chicken lysozyme, transgenic mice, phased nucleosomes, genomic position effects",
author = "Constanze Bonifer and M Huber and N Faust and AE Sippel",
year = "1996",
doi = "10.1615/CritRevEukarGeneExpr.v6.i2-3.90",
language = "English",
volume = "6",
pages = "285--297",
journal = "Critical Reviews in Eukaryotic Gene Expression",
issn = "1045-4403",
publisher = "Begell House",
number = "2-3",

}

RIS

TY - JOUR

T1 - Regulation of Chicken Lysozyme Gene Expression in Transgenic Mice

AU - Bonifer, Constanze

AU - Huber, M

AU - Faust, N

AU - Sippel, AE

PY - 1996

Y1 - 1996

N2 - The chicken lysozyme locus is transcriptionally activated during macrophage differentiation. Each cis-regulatory element has its unique activation stage during cell differentiation, whereby maximal transcriptional activity of the gene is only observed when all cis-elements are active. The complete chicken lysozyme locus is expressed position independently and at a high level in macrophages of transgenic mice. For correct transgene regulation, the cooperation of all cis-regulatory elements is required. These cis-regulatory elements specify the mode of regulation and we observe the same expression pattern of the transgene in the mouse and the endogenous gene in chicken macrophages. This indicates that the transcription factors responsible for chicken lysozyme regulation are highly conserved in evolution. The endogenous mouse lysozyme gene is regulated differently. The chromatin of the lysozyme locus is highly structured in the transcriptionally active, as well as in the inactive state. The transcriptional activation of the lysozyme locus is accompanied by extensive chromatin rearrangements, which are disturbed when one essential cis-regulatory element is deleted and the transgenes are subjects to genomic position effects. Based on these results, we propose that a distinct chromatin architecture of a gene locus is required for its correct activation.

AB - The chicken lysozyme locus is transcriptionally activated during macrophage differentiation. Each cis-regulatory element has its unique activation stage during cell differentiation, whereby maximal transcriptional activity of the gene is only observed when all cis-elements are active. The complete chicken lysozyme locus is expressed position independently and at a high level in macrophages of transgenic mice. For correct transgene regulation, the cooperation of all cis-regulatory elements is required. These cis-regulatory elements specify the mode of regulation and we observe the same expression pattern of the transgene in the mouse and the endogenous gene in chicken macrophages. This indicates that the transcription factors responsible for chicken lysozyme regulation are highly conserved in evolution. The endogenous mouse lysozyme gene is regulated differently. The chromatin of the lysozyme locus is highly structured in the transcriptionally active, as well as in the inactive state. The transcriptional activation of the lysozyme locus is accompanied by extensive chromatin rearrangements, which are disturbed when one essential cis-regulatory element is deleted and the transgenes are subjects to genomic position effects. Based on these results, we propose that a distinct chromatin architecture of a gene locus is required for its correct activation.

KW - chicken lysozyme

KW - transgenic mice

KW - phased nucleosomes

KW - genomic position effects

U2 - 10.1615/CritRevEukarGeneExpr.v6.i2-3.90

DO - 10.1615/CritRevEukarGeneExpr.v6.i2-3.90

M3 - Article

VL - 6

SP - 285

EP - 297

JO - Critical Reviews in Eukaryotic Gene Expression

JF - Critical Reviews in Eukaryotic Gene Expression

SN - 1045-4403

IS - 2-3

ER -