Reduced c-myb expression levels affect hematopoietic development in vitro

Research output: Contribution to journalArticle

Standard

Reduced c-myb expression levels affect hematopoietic development in vitro. / Jieping, C; Clarke, D; Bonifer, Constanze.

In: International journal of hematology, Vol. 85, No. 4, 01.05.2007, p. 312-6.

Research output: Contribution to journalArticle

Harvard

APA

Vancouver

Author

Bibtex

@article{2631fffc57464ace8a25afb7a2105e78,
title = "Reduced c-myb expression levels affect hematopoietic development in vitro",
abstract = "c-myb is an important transcription factor in early hematopoietic development and differentiation. In the study presented here, we asked the question whether a reduction in the level of c-myb expression led to a perturbation of the kinetics of hematopoietic development in differentiating embryonic stem cells. To this end, we induced hematopoietic differentiation of embryonic stem cells in methylcellulose medium in the absence of LIF. Hematopoietic cells formed in embryoid bodies were analyzed by methylcellulose colony assay and the pattern of gene expression was examined by real-time polymerase chain reaction. Our results show that even a small reduction of c-myb expression levels to 55% led to a significant alteration of progenitor development. Erythroid differentiation was particularly affected. However, we do not observe significant alterations in the expression of genes encoding for selected erythroid marker genes.",
keywords = "PU.1 controls ",
author = "C Jieping and D Clarke and Constanze Bonifer",
year = "2007",
month = may,
day = "1",
doi = "10.1532/IJH97.07039",
language = "English",
volume = "85",
pages = "312--6",
journal = "International journal of hematology",
issn = "0925-5710",
publisher = "Springer",
number = "4",

}

RIS

TY - JOUR

T1 - Reduced c-myb expression levels affect hematopoietic development in vitro

AU - Jieping, C

AU - Clarke, D

AU - Bonifer, Constanze

PY - 2007/5/1

Y1 - 2007/5/1

N2 - c-myb is an important transcription factor in early hematopoietic development and differentiation. In the study presented here, we asked the question whether a reduction in the level of c-myb expression led to a perturbation of the kinetics of hematopoietic development in differentiating embryonic stem cells. To this end, we induced hematopoietic differentiation of embryonic stem cells in methylcellulose medium in the absence of LIF. Hematopoietic cells formed in embryoid bodies were analyzed by methylcellulose colony assay and the pattern of gene expression was examined by real-time polymerase chain reaction. Our results show that even a small reduction of c-myb expression levels to 55% led to a significant alteration of progenitor development. Erythroid differentiation was particularly affected. However, we do not observe significant alterations in the expression of genes encoding for selected erythroid marker genes.

AB - c-myb is an important transcription factor in early hematopoietic development and differentiation. In the study presented here, we asked the question whether a reduction in the level of c-myb expression led to a perturbation of the kinetics of hematopoietic development in differentiating embryonic stem cells. To this end, we induced hematopoietic differentiation of embryonic stem cells in methylcellulose medium in the absence of LIF. Hematopoietic cells formed in embryoid bodies were analyzed by methylcellulose colony assay and the pattern of gene expression was examined by real-time polymerase chain reaction. Our results show that even a small reduction of c-myb expression levels to 55% led to a significant alteration of progenitor development. Erythroid differentiation was particularly affected. However, we do not observe significant alterations in the expression of genes encoding for selected erythroid marker genes.

KW - PU.1 controls

U2 - 10.1532/IJH97.07039

DO - 10.1532/IJH97.07039

M3 - Article

C2 - 17483074

VL - 85

SP - 312

EP - 316

JO - International journal of hematology

JF - International journal of hematology

SN - 0925-5710

IS - 4

ER -