Redistribution of protein kinase C during mitogenesis of human B lymphocytes

Research output: Contribution to journalArticlepeer-review

Standard

Redistribution of protein kinase C during mitogenesis of human B lymphocytes. / Guy, Graeme R.; Gordon, John; Walker, Leonie; Michell, Robert H.; Brown, Geoffrey.

In: Biochemical and Biophysical Research Communications, Vol. 135, No. 1, 26.02.1986, p. 146-153.

Research output: Contribution to journalArticlepeer-review

Harvard

APA

Vancouver

Author

Bibtex

@article{f86ce0f9c0d442e4b149b526267573dd,
title = "Redistribution of protein kinase C during mitogenesis of human B lymphocytes",
abstract = "G0 human tonsillar B-lymphocytes were stimulated to divide by the polyclonal mitogen Staphylococcus Aureus Cowan strain 1 (SAC) and by the combined use of 12-O-tetradecanoyl phorbol-13-acetate (TPA) and the calcium ionophore ionomycin. The activities of protein kinase C, which requires Ca++ and phospholipid as co-factors, and a proteolytically cleaved form of this enzyme (protein kinase M), which is independent of calcium and phospholipid control, were determined in soluble and particulate fractions obtained from activated B cells. Treatment of G0 B cells with SAC or TPA together with ionomycin caused redistribution of protein kinase C from the soluble to the particulate fraction where the 80,000-Dalton protein kinase C was cleaved to give rise to a 50,000-Dalton form of the kinase which was also found in the cytoplasm. These data suggest that redistribution and proteolytic cleavage of protein kinase C are key signal transduction events in B cell mitogenesis.",
author = "Guy, {Graeme R.} and John Gordon and Leonie Walker and Michell, {Robert H.} and Geoffrey Brown",
year = "1986",
month = feb,
day = "26",
doi = "10.1016/0006-291X(86)90954-X",
language = "English",
volume = "135",
pages = "146--153",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Redistribution of protein kinase C during mitogenesis of human B lymphocytes

AU - Guy, Graeme R.

AU - Gordon, John

AU - Walker, Leonie

AU - Michell, Robert H.

AU - Brown, Geoffrey

PY - 1986/2/26

Y1 - 1986/2/26

N2 - G0 human tonsillar B-lymphocytes were stimulated to divide by the polyclonal mitogen Staphylococcus Aureus Cowan strain 1 (SAC) and by the combined use of 12-O-tetradecanoyl phorbol-13-acetate (TPA) and the calcium ionophore ionomycin. The activities of protein kinase C, which requires Ca++ and phospholipid as co-factors, and a proteolytically cleaved form of this enzyme (protein kinase M), which is independent of calcium and phospholipid control, were determined in soluble and particulate fractions obtained from activated B cells. Treatment of G0 B cells with SAC or TPA together with ionomycin caused redistribution of protein kinase C from the soluble to the particulate fraction where the 80,000-Dalton protein kinase C was cleaved to give rise to a 50,000-Dalton form of the kinase which was also found in the cytoplasm. These data suggest that redistribution and proteolytic cleavage of protein kinase C are key signal transduction events in B cell mitogenesis.

AB - G0 human tonsillar B-lymphocytes were stimulated to divide by the polyclonal mitogen Staphylococcus Aureus Cowan strain 1 (SAC) and by the combined use of 12-O-tetradecanoyl phorbol-13-acetate (TPA) and the calcium ionophore ionomycin. The activities of protein kinase C, which requires Ca++ and phospholipid as co-factors, and a proteolytically cleaved form of this enzyme (protein kinase M), which is independent of calcium and phospholipid control, were determined in soluble and particulate fractions obtained from activated B cells. Treatment of G0 B cells with SAC or TPA together with ionomycin caused redistribution of protein kinase C from the soluble to the particulate fraction where the 80,000-Dalton protein kinase C was cleaved to give rise to a 50,000-Dalton form of the kinase which was also found in the cytoplasm. These data suggest that redistribution and proteolytic cleavage of protein kinase C are key signal transduction events in B cell mitogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0022516009&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(86)90954-X

DO - 10.1016/0006-291X(86)90954-X

M3 - Article

C2 - 3006678

AN - SCOPUS:0022516009

VL - 135

SP - 146

EP - 153

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -