Recognition of microbial and mammalian phospholipid antigens by NKT cells with diverse TCRs

Research output: Contribution to journalArticlepeer-review

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Recognition of microbial and mammalian phospholipid antigens by NKT cells with diverse TCRs. / Tatituri, Raju V V; Watts, Gerald F M; Bhowruth, Veemal; Barton, Nathaniel; Rothchild, Alissa; Hsu, Fong-Fu; Almeida, Catarina F; Cox, Liam R; Eggeling, Lothar; Cardell, Susanna; Rossjohn, Jamie; Godfrey, Dale I; Behar, Samuel M; Besra, Gurdyal S; Brenner, Michael B; Brigl, Manfred.

In: National Academy of Sciences. Proceedings, Vol. 110, No. 5, 2013, p. 1827-32.

Research output: Contribution to journalArticlepeer-review

Harvard

Tatituri, RVV, Watts, GFM, Bhowruth, V, Barton, N, Rothchild, A, Hsu, F-F, Almeida, CF, Cox, LR, Eggeling, L, Cardell, S, Rossjohn, J, Godfrey, DI, Behar, SM, Besra, GS, Brenner, MB & Brigl, M 2013, 'Recognition of microbial and mammalian phospholipid antigens by NKT cells with diverse TCRs', National Academy of Sciences. Proceedings, vol. 110, no. 5, pp. 1827-32. https://doi.org/10.1073/pnas.1220601110

APA

Tatituri, R. V. V., Watts, G. F. M., Bhowruth, V., Barton, N., Rothchild, A., Hsu, F-F., Almeida, C. F., Cox, L. R., Eggeling, L., Cardell, S., Rossjohn, J., Godfrey, D. I., Behar, S. M., Besra, G. S., Brenner, M. B., & Brigl, M. (2013). Recognition of microbial and mammalian phospholipid antigens by NKT cells with diverse TCRs. National Academy of Sciences. Proceedings, 110(5), 1827-32. https://doi.org/10.1073/pnas.1220601110

Vancouver

Author

Tatituri, Raju V V ; Watts, Gerald F M ; Bhowruth, Veemal ; Barton, Nathaniel ; Rothchild, Alissa ; Hsu, Fong-Fu ; Almeida, Catarina F ; Cox, Liam R ; Eggeling, Lothar ; Cardell, Susanna ; Rossjohn, Jamie ; Godfrey, Dale I ; Behar, Samuel M ; Besra, Gurdyal S ; Brenner, Michael B ; Brigl, Manfred. / Recognition of microbial and mammalian phospholipid antigens by NKT cells with diverse TCRs. In: National Academy of Sciences. Proceedings. 2013 ; Vol. 110, No. 5. pp. 1827-32.

Bibtex

@article{bdb16937bdd042c3ac6c66bf1c1899cf,
title = "Recognition of microbial and mammalian phospholipid antigens by NKT cells with diverse TCRs",
abstract = "CD1d-restricted natural killer T (NKT) cells include two major subgroups. The most widely studied are Vα14Jα18(+) invariant NKT (iNKT) cells that recognize the prototypical α-galactosylceramide antigen, whereas the other major group uses diverse T-cell receptor (TCR) α-and β-chains, does not recognize α-galactosylceramide, and is referred to as diverse NKT (dNKT) cells. dNKT cells play important roles during infection and autoimmunity, but the antigens they recognize remain poorly understood. Here, we identified phosphatidylglycerol (PG), diphosphatidylglycerol (DPG, or cardiolipin), and phosphatidylinositol from Mycobacterium tuberculosis or Corynebacterium glutamicum as microbial antigens that stimulated various dNKT, but not iNKT, hybridomas. dNKT hybridomas showed distinct reactivities for diverse antigens. Stimulation of dNKT hybridomas by microbial PG was independent of Toll-like receptor-mediated signaling by antigen-presenting cells and required lipid uptake and/or processing. Furthermore, microbial PG bound to CD1d molecules and plate-bound PG/CD1d complexes stimulated dNKT hybridomas, indicating direct recognition by the dNKT cell TCR. Interestingly, despite structural differences in acyl chain composition between microbial and mammalian PG and DPG, lipids from both sources stimulated dNKT hybridomas, suggesting that presentation of microbial lipids and enhanced availability of stimulatory self-lipids may both contribute to dNKT cell activation during infection.",
author = "Tatituri, {Raju V V} and Watts, {Gerald F M} and Veemal Bhowruth and Nathaniel Barton and Alissa Rothchild and Fong-Fu Hsu and Almeida, {Catarina F} and Cox, {Liam R} and Lothar Eggeling and Susanna Cardell and Jamie Rossjohn and Godfrey, {Dale I} and Behar, {Samuel M} and Besra, {Gurdyal S} and Brenner, {Michael B} and Manfred Brigl",
year = "2013",
doi = "10.1073/pnas.1220601110",
language = "English",
volume = "110",
pages = "1827--32",
journal = "Proceedings of the National Academy of Sciences",
issn = "1091-6490",
publisher = "National Academy of Sciences",
number = "5",

}

RIS

TY - JOUR

T1 - Recognition of microbial and mammalian phospholipid antigens by NKT cells with diverse TCRs

AU - Tatituri, Raju V V

AU - Watts, Gerald F M

AU - Bhowruth, Veemal

AU - Barton, Nathaniel

AU - Rothchild, Alissa

AU - Hsu, Fong-Fu

AU - Almeida, Catarina F

AU - Cox, Liam R

AU - Eggeling, Lothar

AU - Cardell, Susanna

AU - Rossjohn, Jamie

AU - Godfrey, Dale I

AU - Behar, Samuel M

AU - Besra, Gurdyal S

AU - Brenner, Michael B

AU - Brigl, Manfred

PY - 2013

Y1 - 2013

N2 - CD1d-restricted natural killer T (NKT) cells include two major subgroups. The most widely studied are Vα14Jα18(+) invariant NKT (iNKT) cells that recognize the prototypical α-galactosylceramide antigen, whereas the other major group uses diverse T-cell receptor (TCR) α-and β-chains, does not recognize α-galactosylceramide, and is referred to as diverse NKT (dNKT) cells. dNKT cells play important roles during infection and autoimmunity, but the antigens they recognize remain poorly understood. Here, we identified phosphatidylglycerol (PG), diphosphatidylglycerol (DPG, or cardiolipin), and phosphatidylinositol from Mycobacterium tuberculosis or Corynebacterium glutamicum as microbial antigens that stimulated various dNKT, but not iNKT, hybridomas. dNKT hybridomas showed distinct reactivities for diverse antigens. Stimulation of dNKT hybridomas by microbial PG was independent of Toll-like receptor-mediated signaling by antigen-presenting cells and required lipid uptake and/or processing. Furthermore, microbial PG bound to CD1d molecules and plate-bound PG/CD1d complexes stimulated dNKT hybridomas, indicating direct recognition by the dNKT cell TCR. Interestingly, despite structural differences in acyl chain composition between microbial and mammalian PG and DPG, lipids from both sources stimulated dNKT hybridomas, suggesting that presentation of microbial lipids and enhanced availability of stimulatory self-lipids may both contribute to dNKT cell activation during infection.

AB - CD1d-restricted natural killer T (NKT) cells include two major subgroups. The most widely studied are Vα14Jα18(+) invariant NKT (iNKT) cells that recognize the prototypical α-galactosylceramide antigen, whereas the other major group uses diverse T-cell receptor (TCR) α-and β-chains, does not recognize α-galactosylceramide, and is referred to as diverse NKT (dNKT) cells. dNKT cells play important roles during infection and autoimmunity, but the antigens they recognize remain poorly understood. Here, we identified phosphatidylglycerol (PG), diphosphatidylglycerol (DPG, or cardiolipin), and phosphatidylinositol from Mycobacterium tuberculosis or Corynebacterium glutamicum as microbial antigens that stimulated various dNKT, but not iNKT, hybridomas. dNKT hybridomas showed distinct reactivities for diverse antigens. Stimulation of dNKT hybridomas by microbial PG was independent of Toll-like receptor-mediated signaling by antigen-presenting cells and required lipid uptake and/or processing. Furthermore, microbial PG bound to CD1d molecules and plate-bound PG/CD1d complexes stimulated dNKT hybridomas, indicating direct recognition by the dNKT cell TCR. Interestingly, despite structural differences in acyl chain composition between microbial and mammalian PG and DPG, lipids from both sources stimulated dNKT hybridomas, suggesting that presentation of microbial lipids and enhanced availability of stimulatory self-lipids may both contribute to dNKT cell activation during infection.

U2 - 10.1073/pnas.1220601110

DO - 10.1073/pnas.1220601110

M3 - Article

C2 - 23307809

VL - 110

SP - 1827

EP - 1832

JO - Proceedings of the National Academy of Sciences

JF - Proceedings of the National Academy of Sciences

SN - 1091-6490

IS - 5

ER -