TY - JOUR
T1 - Receptor-mediated gene transfer by phage-display vectors: applications in functional genomics and gene therapy
AU - Larocca, D
AU - Baird, Andrew
PY - 2001/8/1
Y1 - 2001/8/1
N2 - Recent studies have demonstrated targeted gene-delivery to mammalian cells using modified phage-display vectors. Specificity is determined by the choice of the genetically displayed targeting ligand. Without targeting, phage particles have virtually no tropism for mammalian cells. Thus, novel ligands can be selected from phage libraries by their ability to deliver a reporter gene to targeted cells. Together with advances in cDNA display technologies, these findings offer new opportunities for the use of phage-display technology in functional genomics. In addition, targeted phage particles have potential as alternative gene therapy vectors that can be further improved using directed evolution.
AB - Recent studies have demonstrated targeted gene-delivery to mammalian cells using modified phage-display vectors. Specificity is determined by the choice of the genetically displayed targeting ligand. Without targeting, phage particles have virtually no tropism for mammalian cells. Thus, novel ligands can be selected from phage libraries by their ability to deliver a reporter gene to targeted cells. Together with advances in cDNA display technologies, these findings offer new opportunities for the use of phage-display technology in functional genomics. In addition, targeted phage particles have potential as alternative gene therapy vectors that can be further improved using directed evolution.
UR - http://www.scopus.com/inward/record.url?scp=0035424184&partnerID=8YFLogxK
U2 - 10.1016/S1359-6446(01)01837-2
DO - 10.1016/S1359-6446(01)01837-2
M3 - Article
C2 - 11470588
VL - 6
SP - 793
EP - 801
JO - Drug Discovery Today
JF - Drug Discovery Today
IS - 15
ER -