Receptor-mediated gene transfer by phage-display vectors: applications in functional genomics and gene therapy

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

Abstract

Recent studies have demonstrated targeted gene-delivery to mammalian cells using modified phage-display vectors. Specificity is determined by the choice of the genetically displayed targeting ligand. Without targeting, phage particles have virtually no tropism for mammalian cells. Thus, novel ligands can be selected from phage libraries by their ability to deliver a reporter gene to targeted cells. Together with advances in cDNA display technologies, these findings offer new opportunities for the use of phage-display technology in functional genomics. In addition, targeted phage particles have potential as alternative gene therapy vectors that can be further improved using directed evolution.

Details

Original languageEnglish
Pages (from-to)793-801
Number of pages9
JournalDrug Discovery Today
Volume6
Issue number15
Publication statusPublished - 1 Aug 2001