Real time monitoring of BMP smads transcriptional activity during mouse development

Research output: Contribution to journalArticle

Authors

  • Susana M Chuva de Sousa Lopes
  • Monika Bialecka
  • Sophie de Boer
  • An Zwijsen
  • Christine L. Mummery

Colleges, School and Institutes

External organisations

  • Hubrecht Laboratory KNAW (Netherlands Institute for Developmental Biology)
  • Leiden University Medical Center - LUMC
  • KU Leuven Univ

Abstract

Bone morphogenetic protein (BMP) signaling is a key pathway in the patterning and development of organisms as diverse as fruit fly and humans. However, the determination of net BMP signaling, paramount to understanding organogenesis, is limited to the analysis of fixed material. We generated a transgenic mouse that reports the transcriptional response of BMP Smad activation by coupling a well established BMP response element (BRE), isolated from the Id1 promoter, to green fluorescent protein (BRE:gfp). We monitored BMP Smad transcriptional activity in fresh and fixed BRE:gfp embryos. GFP expression was observed where expected on the basis of known signaling sites, but also in specific cell populations in which BMP signaling had been implicated but not directly demonstrated. Deletion of Smad5 reduced GFP in vivo as expected. The BRE:gfp transgenic mice are a novel tool, which will facilitate the identification of specific BMP Smad responsive cell types and allow BMP Smad signaling to be monitored in real time, supporting studies in development and disease.

Details

Original languageEnglish
Pages (from-to)335-346
Number of pages12
JournalGenesis
Volume46
Issue number7
Publication statusPublished - 1 Jul 2008

Keywords

  • Bone morphogenetic protein (BMP), Embryonic development, GFP, Mice, Smads, Transgene

ASJC Scopus subject areas