Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite

Research output: Contribution to journalArticlepeer-review

Standard

Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite. / Sun, Xiaolong; Lacina, Karel; Ramsamy, Elena C.; Flower, Stephen E.; Fossey, John S.; Qian, Xuhong; Anslyn, Eric V.; Bull, Steven D.; James, Tony D.

In: Chemical Science, Vol. 6, No. 5, 01.05.2015, p. 2963-2967.

Research output: Contribution to journalArticlepeer-review

Harvard

Sun, X, Lacina, K, Ramsamy, EC, Flower, SE, Fossey, JS, Qian, X, Anslyn, EV, Bull, SD & James, TD 2015, 'Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite', Chemical Science, vol. 6, no. 5, pp. 2963-2967. https://doi.org/10.1039/c4sc03983a

APA

Sun, X., Lacina, K., Ramsamy, E. C., Flower, S. E., Fossey, J. S., Qian, X., Anslyn, E. V., Bull, S. D., & James, T. D. (2015). Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite. Chemical Science, 6(5), 2963-2967. https://doi.org/10.1039/c4sc03983a

Vancouver

Author

Sun, Xiaolong ; Lacina, Karel ; Ramsamy, Elena C. ; Flower, Stephen E. ; Fossey, John S. ; Qian, Xuhong ; Anslyn, Eric V. ; Bull, Steven D. ; James, Tony D. / Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite. In: Chemical Science. 2015 ; Vol. 6, No. 5. pp. 2963-2967.

Bibtex

@article{6a21f45992a64f7d9eae3611562a9606,
title = "Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite",
abstract = "Using the self-assembly of aromatic boronic acids with Alizarin Red S (ARS), we developed a new chemosensor for the selective detection of peroxynitrite. Phenylboronic acid (PBA), benzoboroxole (BBA) and 2-(N,N-dimethylaminomethyl)phenylboronic acid (NBA) were employed to bind with ARS to form the complex probes. In particular, the ARS-NBA system with a high binding affinity can preferably react with peroxynitrite over hydrogen peroxide and other ROS/RNS due to the protection of the boron via the solvent-insertion B-N interaction. Our simple system produces a visible colorimetric change and on-off fluorescence response towards peroxynitrite. By coupling a chemical reaction that leads to an indicator displacement, we have developed a new sensing strategy, referred to herein as RIA (Reaction-based Indicator displacement Assay).",
author = "Xiaolong Sun and Karel Lacina and Ramsamy, {Elena C.} and Flower, {Stephen E.} and Fossey, {John S.} and Xuhong Qian and Anslyn, {Eric V.} and Bull, {Steven D.} and James, {Tony D.}",
year = "2015",
month = may,
day = "1",
doi = "10.1039/c4sc03983a",
language = "English",
volume = "6",
pages = "2963--2967",
journal = "Chemical Science",
issn = "2041-6520",
publisher = "Royal Society of Chemistry",
number = "5",

}

RIS

TY - JOUR

T1 - Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite

AU - Sun, Xiaolong

AU - Lacina, Karel

AU - Ramsamy, Elena C.

AU - Flower, Stephen E.

AU - Fossey, John S.

AU - Qian, Xuhong

AU - Anslyn, Eric V.

AU - Bull, Steven D.

AU - James, Tony D.

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Using the self-assembly of aromatic boronic acids with Alizarin Red S (ARS), we developed a new chemosensor for the selective detection of peroxynitrite. Phenylboronic acid (PBA), benzoboroxole (BBA) and 2-(N,N-dimethylaminomethyl)phenylboronic acid (NBA) were employed to bind with ARS to form the complex probes. In particular, the ARS-NBA system with a high binding affinity can preferably react with peroxynitrite over hydrogen peroxide and other ROS/RNS due to the protection of the boron via the solvent-insertion B-N interaction. Our simple system produces a visible colorimetric change and on-off fluorescence response towards peroxynitrite. By coupling a chemical reaction that leads to an indicator displacement, we have developed a new sensing strategy, referred to herein as RIA (Reaction-based Indicator displacement Assay).

AB - Using the self-assembly of aromatic boronic acids with Alizarin Red S (ARS), we developed a new chemosensor for the selective detection of peroxynitrite. Phenylboronic acid (PBA), benzoboroxole (BBA) and 2-(N,N-dimethylaminomethyl)phenylboronic acid (NBA) were employed to bind with ARS to form the complex probes. In particular, the ARS-NBA system with a high binding affinity can preferably react with peroxynitrite over hydrogen peroxide and other ROS/RNS due to the protection of the boron via the solvent-insertion B-N interaction. Our simple system produces a visible colorimetric change and on-off fluorescence response towards peroxynitrite. By coupling a chemical reaction that leads to an indicator displacement, we have developed a new sensing strategy, referred to herein as RIA (Reaction-based Indicator displacement Assay).

UR - http://www.scopus.com/inward/record.url?scp=84928137681&partnerID=8YFLogxK

U2 - 10.1039/c4sc03983a

DO - 10.1039/c4sc03983a

M3 - Article

AN - SCOPUS:84928137681

VL - 6

SP - 2963

EP - 2967

JO - Chemical Science

JF - Chemical Science

SN - 2041-6520

IS - 5

ER -