Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite

Research output: Contribution to journalArticlepeer-review


  • Xiaolong Sun
  • Karel Lacina
  • Elena C. Ramsamy
  • Stephen E. Flower
  • Xuhong Qian
  • Eric V. Anslyn
  • Steven D. Bull
  • Tony D. James

Colleges, School and Institutes

External organisations

  • University of Nottingham
  • City University of New York
  • University of Bath
  • CEITEC, Masaryk University
  • East China University of Science and Technology
  • University of Texas at Austin


Using the self-assembly of aromatic boronic acids with Alizarin Red S (ARS), we developed a new chemosensor for the selective detection of peroxynitrite. Phenylboronic acid (PBA), benzoboroxole (BBA) and 2-(N,N-dimethylaminomethyl)phenylboronic acid (NBA) were employed to bind with ARS to form the complex probes. In particular, the ARS-NBA system with a high binding affinity can preferably react with peroxynitrite over hydrogen peroxide and other ROS/RNS due to the protection of the boron via the solvent-insertion B-N interaction. Our simple system produces a visible colorimetric change and on-off fluorescence response towards peroxynitrite. By coupling a chemical reaction that leads to an indicator displacement, we have developed a new sensing strategy, referred to herein as RIA (Reaction-based Indicator displacement Assay).


Original languageEnglish
Pages (from-to)2963-2967
Number of pages5
JournalChemical Science
Issue number5
Early online date6 Mar 2015
Publication statusPublished - 1 May 2015

ASJC Scopus subject areas