Rapid resistome mapping using nanopore sequencing

Research output: Contribution to journalArticlepeer-review

Authors

  • Eric van der Helm
  • Lejla Imamovic
  • Mostafa M Hashim Ellabaan
  • Anna Koza
  • Morten O A Sommer

Colleges, School and Institutes

External organisations

  • Technical University of Denmark

Abstract

The emergence of antibiotic resistance in human pathogens has become a major threat to modern medicine. The outcome of antibiotic treatment can be affected by the composition of the gut. Accordingly, knowledge of the gut resistome composition could enable more effective and individualized treatment of bacterial infections. Yet, rapid workflows for resistome characterization are lacking. To address this challenge we developed the poreFUME workflow that deploys functional metagenomic selections and nanopore sequencing to resistome mapping. We demonstrate the approach by functionally characterizing the gut resistome of an ICU (intensive care unit) patient. The accuracy of the poreFUME pipeline is with >97% sufficient for the annotation of antibiotic resistance genes. The poreFUME pipeline provides a promising approach for efficient resistome profiling that could inform antibiotic treatment decisions in the future.

Details

Original languageEnglish
Article numbere61
JournalNucleic Acids Research
Volume45
Issue number8
Early online date6 Jan 2017
Publication statusPublished - 5 May 2017

Keywords

  • Journal Article

Sustainable Development Goals