Rapid optofluidic detection of biomarkers for traumatic brain injury via surface-enhanced Raman spectroscopy

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@article{bbf86d374d754797b423158b2f189fde,
title = "Rapid optofluidic detection of biomarkers for traumatic brain injury via surface-enhanced Raman spectroscopy",
abstract = "Current technologies for the point-of-care diagnosis of traumatic brain injury (TBI) lack sensitivity, require specialist handling or involve complicated and costly procedures. Here, we report the development and testing of an optofluidic device for the rapid and label-free detection, via surface-enhanced Raman scattering (SERS), of picomolar concentrations of biomarkers for TBI in biofluids. The SERS-active substrate of the device consists of electrohydrodynamically fabricated submicrometre pillars covered with a plasmon-active nanometric gold layer, integrated in an optofluidic chip. We show that the device can detect N-acetylasparate in finger-prick blood samples from patients with TBI, and that the biomarker is released immediately from the central nervous system after TBI. The simplicity, sensitivity and robustness of SERS-integrated optofluidic technology might eventually help the triaging of TBI patients and assist clinical decision making at point-of-care settings.",
author = "Jonathan Rickard and {Di Pietro}, Valentina and David Smith and David Davies and Tony Belli and {Goldberg Oppenheimer}, Pola",
year = "2020",
month = jun,
doi = "10.1038/s41551-019-0510-4",
language = "English",
volume = "4",
pages = "610--623",
journal = "Nature Biomedical Engineering",
issn = "2157-846X",
publisher = "Nature Publishing Group",
number = "6",

}

RIS

TY - JOUR

T1 - Rapid optofluidic detection of biomarkers for traumatic brain injury via surface-enhanced Raman spectroscopy

AU - Rickard, Jonathan

AU - Di Pietro, Valentina

AU - Smith, David

AU - Davies, David

AU - Belli, Tony

AU - Goldberg Oppenheimer, Pola

PY - 2020/6

Y1 - 2020/6

N2 - Current technologies for the point-of-care diagnosis of traumatic brain injury (TBI) lack sensitivity, require specialist handling or involve complicated and costly procedures. Here, we report the development and testing of an optofluidic device for the rapid and label-free detection, via surface-enhanced Raman scattering (SERS), of picomolar concentrations of biomarkers for TBI in biofluids. The SERS-active substrate of the device consists of electrohydrodynamically fabricated submicrometre pillars covered with a plasmon-active nanometric gold layer, integrated in an optofluidic chip. We show that the device can detect N-acetylasparate in finger-prick blood samples from patients with TBI, and that the biomarker is released immediately from the central nervous system after TBI. The simplicity, sensitivity and robustness of SERS-integrated optofluidic technology might eventually help the triaging of TBI patients and assist clinical decision making at point-of-care settings.

AB - Current technologies for the point-of-care diagnosis of traumatic brain injury (TBI) lack sensitivity, require specialist handling or involve complicated and costly procedures. Here, we report the development and testing of an optofluidic device for the rapid and label-free detection, via surface-enhanced Raman scattering (SERS), of picomolar concentrations of biomarkers for TBI in biofluids. The SERS-active substrate of the device consists of electrohydrodynamically fabricated submicrometre pillars covered with a plasmon-active nanometric gold layer, integrated in an optofluidic chip. We show that the device can detect N-acetylasparate in finger-prick blood samples from patients with TBI, and that the biomarker is released immediately from the central nervous system after TBI. The simplicity, sensitivity and robustness of SERS-integrated optofluidic technology might eventually help the triaging of TBI patients and assist clinical decision making at point-of-care settings.

UR - http://www.scopus.com/inward/record.url?scp=85079138137&partnerID=8YFLogxK

U2 - 10.1038/s41551-019-0510-4

DO - 10.1038/s41551-019-0510-4

M3 - Article

C2 - 32015408

VL - 4

SP - 610

EP - 623

JO - Nature Biomedical Engineering

JF - Nature Biomedical Engineering

SN - 2157-846X

IS - 6

ER -