Quinolone-resistant Salmonella typhi in Viet Nam: molecular basis of resistance and clinical response to treatment

J Wain; N T Hoa; N T Chinh; H Vinh; M J Everett; T S Diep; N P Day; T Solomon; N J White; L J Piddock; C M Parry

Quinolone-resistant Salmonella typhi in Viet Nam: molecular basis of resistance and clinical response to treatment

J Wain, N T Hoa, N T Chinh, H Vinh, M J Everett, T S Diep, N P Day, T Solomon, N J White, L J Piddock, C M Parry

Immunology and Immunotherapy

Research output: Contribution to journal › Article › peer-review

268 Citations (Scopus)

Abstract

Nalidixic acid-resistant Salmonella typhi (NARST) was first isolated in Viet Nam in 1993. Analysis of the quinolone resistance-determining region of gyrA in 20 NARST isolates by polymerase chain reaction and single-stranded conformational polymorphism yielded two novel patterns: pattern II corresponding to a point mutation at nucleotide 87 Asp-->Gly (n = 17), and pattern III corresponding to a point mutation at nucleotide 83 Ser-->Phe (n = 3). In trials of short-course ofloxacin therapy for uncomplicated typhoid, 117 (78%) of 150 patients were infected with multidrug-resistant S. typhi, 18 (15%) of which were NARST. The median time to fever clearance was 156 hours (range, 30-366 hours) for patients infected with NARST and 84 hours (range, 12-378 hours) for those infected with nalidixic acid-susceptible strains (P

Original language	English
Pages (from-to)	1404-10
Number of pages	7
Journal	Clinical Infectious Diseases
Volume	25
Issue number	6
Publication status	Published - Dec 1997

Keywords

4-Quinolones
Anti-Infective Agents
Child
DNA Gyrase
DNA Topoisomerases, Type II
Drug Resistance, Microbial
Drug Resistance, Multiple
Female
Humans
Male
Microbial Sensitivity Tests
Prospective Studies
Salmonella typhi
Sequence Analysis, DNA
Typhoid Fever
Vietnam

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{67f97073002b45af966e59e23311b985,

title = "Quinolone-resistant Salmonella typhi in Viet Nam: molecular basis of resistance and clinical response to treatment",

abstract = "Nalidixic acid-resistant Salmonella typhi (NARST) was first isolated in Viet Nam in 1993. Analysis of the quinolone resistance-determining region of gyrA in 20 NARST isolates by polymerase chain reaction and single-stranded conformational polymorphism yielded two novel patterns: pattern II corresponding to a point mutation at nucleotide 87 Asp-->Gly (n = 17), and pattern III corresponding to a point mutation at nucleotide 83 Ser-->Phe (n = 3). In trials of short-course ofloxacin therapy for uncomplicated typhoid, 117 (78%) of 150 patients were infected with multidrug-resistant S. typhi, 18 (15%) of which were NARST. The median time to fever clearance was 156 hours (range, 30-366 hours) for patients infected with NARST and 84 hours (range, 12-378 hours) for those infected with nalidixic acid-susceptible strains (P ",

keywords = "4-Quinolones, Anti-Infective Agents, Child, DNA Gyrase, DNA Topoisomerases, Type II, Drug Resistance, Microbial, Drug Resistance, Multiple, Female, Humans, Male, Microbial Sensitivity Tests, Prospective Studies, Salmonella typhi, Sequence Analysis, DNA, Typhoid Fever, Vietnam",

author = "J Wain and Hoa, {N T} and Chinh, {N T} and H Vinh and Everett, {M J} and Diep, {T S} and Day, {N P} and T Solomon and White, {N J} and Piddock, {L J} and Parry, {C M}",

year = "1997",

month = dec,

language = "English",

volume = "25",

pages = "1404--10",

journal = "Clinical Infectious Diseases",

issn = "1058-4838",

publisher = "Oxford University Press",

number = "6",

}

TY - JOUR

T1 - Quinolone-resistant Salmonella typhi in Viet Nam

T2 - molecular basis of resistance and clinical response to treatment

AU - Wain, J

AU - Hoa, N T

AU - Chinh, N T

AU - Vinh, H

AU - Everett, M J

AU - Diep, T S

AU - Day, N P

AU - Solomon, T

AU - White, N J

AU - Piddock, L J

AU - Parry, C M

PY - 1997/12

Y1 - 1997/12

N2 - Nalidixic acid-resistant Salmonella typhi (NARST) was first isolated in Viet Nam in 1993. Analysis of the quinolone resistance-determining region of gyrA in 20 NARST isolates by polymerase chain reaction and single-stranded conformational polymorphism yielded two novel patterns: pattern II corresponding to a point mutation at nucleotide 87 Asp-->Gly (n = 17), and pattern III corresponding to a point mutation at nucleotide 83 Ser-->Phe (n = 3). In trials of short-course ofloxacin therapy for uncomplicated typhoid, 117 (78%) of 150 patients were infected with multidrug-resistant S. typhi, 18 (15%) of which were NARST. The median time to fever clearance was 156 hours (range, 30-366 hours) for patients infected with NARST and 84 hours (range, 12-378 hours) for those infected with nalidixic acid-susceptible strains (P

AB - Nalidixic acid-resistant Salmonella typhi (NARST) was first isolated in Viet Nam in 1993. Analysis of the quinolone resistance-determining region of gyrA in 20 NARST isolates by polymerase chain reaction and single-stranded conformational polymorphism yielded two novel patterns: pattern II corresponding to a point mutation at nucleotide 87 Asp-->Gly (n = 17), and pattern III corresponding to a point mutation at nucleotide 83 Ser-->Phe (n = 3). In trials of short-course ofloxacin therapy for uncomplicated typhoid, 117 (78%) of 150 patients were infected with multidrug-resistant S. typhi, 18 (15%) of which were NARST. The median time to fever clearance was 156 hours (range, 30-366 hours) for patients infected with NARST and 84 hours (range, 12-378 hours) for those infected with nalidixic acid-susceptible strains (P

KW - 4-Quinolones

KW - Anti-Infective Agents

KW - Child

KW - DNA Gyrase

KW - DNA Topoisomerases, Type II

KW - Drug Resistance, Microbial

KW - Drug Resistance, Multiple

KW - Female

KW - Humans

KW - Male

KW - Microbial Sensitivity Tests

KW - Prospective Studies

KW - Salmonella typhi

KW - Sequence Analysis, DNA

KW - Typhoid Fever

KW - Vietnam

M3 - Article

C2 - 9431387

SN - 1058-4838

VL - 25

SP - 1404

EP - 1410

JO - Clinical Infectious Diseases

JF - Clinical Infectious Diseases

IS - 6

ER -

Quinolone-resistant Salmonella typhi in Viet Nam: molecular basis of resistance and clinical response to treatment

Abstract

Keywords

UN SDGs

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