Quinolone resistance in veterinary isolates of Salmonella

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Quinolone resistance in veterinary isolates of Salmonella. / Griggs, D J; Hall, M C; Jin, Y F; Piddock, L J.

In: Journal of Antimicrobial Chemotherapy, Vol. 33, No. 6, 06.1994, p. 1173-89.

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@article{d00f3acdd91f4442b7c72152c1068d10,
title = "Quinolone resistance in veterinary isolates of Salmonella",
abstract = "Twenty-seven nalidixic acid-resistant (MIC > or = 256 mg/L) isolates of salmonella from veterinary sources were also less susceptible to fluoroquinolones (range of MICs of ciprofloxacin, 0.12-2 mg/L). Six isolates were cross-resistant to one or more chemically unrelated antibacterial agents. The concentrations of enrofloxacin that inhibited DNA synthesis by 50% were similar to the MIC values for 23 of 27 isolates, suggesting a mutation in gyrA. Insertion of pNJR3-2 (gyrA) in nine of 20 isolates increased susceptibility to quinolones, suggesting that resistance was due to mutation in gyrA. Five of 27 isolates had reduced levels of accumulation of enrofloxacin. Two of the five also had increased susceptibility to quinolones when pNJR3-2 was introduced. None of the outer membrane protein profiles of the resistant isolates differed from those of sensitive control strains. Three of 27 isolates had differences in lipopolysaccharide profiles compared to control strains. Although the MIC of ciprofloxacin was less than the recommended UK break point concentrations for most isolates, the increasing incidence of quinolone-resistance in salmonella from veterinary sources is a matter of concern.",
keywords = "Animals, Anti-Infective Agents, Chickens, Ciprofloxacin, Conjugation, Genetic, DNA, Bacterial, Drug Resistance, Microbial, Fluoroquinolones, Lipopolysaccharides, Microbial Sensitivity Tests, Mutation, Nalidixic Acid, Poultry Diseases, Quinolones, Salmonella, Salmonella Infections, Animal, Turkeys",
author = "Griggs, {D J} and Hall, {M C} and Jin, {Y F} and Piddock, {L J}",
year = "1994",
month = jun,
language = "English",
volume = "33",
pages = "1173--89",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "6",

}

RIS

TY - JOUR

T1 - Quinolone resistance in veterinary isolates of Salmonella

AU - Griggs, D J

AU - Hall, M C

AU - Jin, Y F

AU - Piddock, L J

PY - 1994/6

Y1 - 1994/6

N2 - Twenty-seven nalidixic acid-resistant (MIC > or = 256 mg/L) isolates of salmonella from veterinary sources were also less susceptible to fluoroquinolones (range of MICs of ciprofloxacin, 0.12-2 mg/L). Six isolates were cross-resistant to one or more chemically unrelated antibacterial agents. The concentrations of enrofloxacin that inhibited DNA synthesis by 50% were similar to the MIC values for 23 of 27 isolates, suggesting a mutation in gyrA. Insertion of pNJR3-2 (gyrA) in nine of 20 isolates increased susceptibility to quinolones, suggesting that resistance was due to mutation in gyrA. Five of 27 isolates had reduced levels of accumulation of enrofloxacin. Two of the five also had increased susceptibility to quinolones when pNJR3-2 was introduced. None of the outer membrane protein profiles of the resistant isolates differed from those of sensitive control strains. Three of 27 isolates had differences in lipopolysaccharide profiles compared to control strains. Although the MIC of ciprofloxacin was less than the recommended UK break point concentrations for most isolates, the increasing incidence of quinolone-resistance in salmonella from veterinary sources is a matter of concern.

AB - Twenty-seven nalidixic acid-resistant (MIC > or = 256 mg/L) isolates of salmonella from veterinary sources were also less susceptible to fluoroquinolones (range of MICs of ciprofloxacin, 0.12-2 mg/L). Six isolates were cross-resistant to one or more chemically unrelated antibacterial agents. The concentrations of enrofloxacin that inhibited DNA synthesis by 50% were similar to the MIC values for 23 of 27 isolates, suggesting a mutation in gyrA. Insertion of pNJR3-2 (gyrA) in nine of 20 isolates increased susceptibility to quinolones, suggesting that resistance was due to mutation in gyrA. Five of 27 isolates had reduced levels of accumulation of enrofloxacin. Two of the five also had increased susceptibility to quinolones when pNJR3-2 was introduced. None of the outer membrane protein profiles of the resistant isolates differed from those of sensitive control strains. Three of 27 isolates had differences in lipopolysaccharide profiles compared to control strains. Although the MIC of ciprofloxacin was less than the recommended UK break point concentrations for most isolates, the increasing incidence of quinolone-resistance in salmonella from veterinary sources is a matter of concern.

KW - Animals

KW - Anti-Infective Agents

KW - Chickens

KW - Ciprofloxacin

KW - Conjugation, Genetic

KW - DNA, Bacterial

KW - Drug Resistance, Microbial

KW - Fluoroquinolones

KW - Lipopolysaccharides

KW - Microbial Sensitivity Tests

KW - Mutation

KW - Nalidixic Acid

KW - Poultry Diseases

KW - Quinolones

KW - Salmonella

KW - Salmonella Infections, Animal

KW - Turkeys

M3 - Article

C2 - 7928811

VL - 33

SP - 1173

EP - 1189

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 6

ER -