Quantitative Analysis of Three-Dimensional Fluorescence Localization Microscopy Data
Research output: Contribution to journal › Article
Colleges, School and Institutes
Identifying the three-dimensional molecular organization of subcellular organelles in intact cells has been challenging to date. Here we present an analysis approach for three-dimensional localization microscopy that can not only identify subcellular objects below the diffraction limit but also quantify their shape and volume. This approach is particularly useful to map the topography of the plasma membrane and measure protein distribution within an undulating membrane.
|Publication status||Published - 16 Jul 2013|
- OPTICAL RECONSTRUCTION MICROSCOPY, PROTEIN HETEROGENEITY, DIFFRACTION-LIMIT, SURFACE, CELLS, PALM