Quantitative Analysis of Three-Dimensional Fluorescence Localization Microscopy Data

Research output: Contribution to journalArticle

Authors

  • David J. Williamson
  • Lies Boelen
  • Astrid Magenau
  • Jeremie Rossy
  • Katharina Gaus

Colleges, School and Institutes

Abstract

Identifying the three-dimensional molecular organization of subcellular organelles in intact cells has been challenging to date. Here we present an analysis approach for three-dimensional localization microscopy that can not only identify subcellular objects below the diffraction limit but also quantify their shape and volume. This approach is particularly useful to map the topography of the plasma membrane and measure protein distribution within an undulating membrane.

Details

Original languageEnglish
Pages (from-to)L05-L07
JournalBiophysical Journal
Volume105
Issue number2
Publication statusPublished - 16 Jul 2013

Keywords

  • OPTICAL RECONSTRUCTION MICROSCOPY, PROTEIN HETEROGENEITY, DIFFRACTION-LIMIT, SURFACE, CELLS, PALM