PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element

AK Ebralidze; FC Guibal; U Steidl; P Zhang; S Lee; B Bartholdy; MA Jorda; V Petkova; F Rosenbauer; G Huang; T Dayaram; J Klupp; KB O'Brien; B Will; Maarten Hoogenkamp; KLB Borden; Constanze Bonifer; DG Tenen

doi:10.1101/gad.1654808

PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element

AK Ebralidze, FC Guibal, U Steidl, P Zhang, S Lee, B Bartholdy, MA Jorda, V Petkova, F Rosenbauer, G Huang, T Dayaram, J Klupp, KB O'Brien, B Will, Maarten Hoogenkamp, KLB Borden, Constanze Bonifer, DG Tenen

Cancer and Genomic Sciences

Research output: Contribution to journal › Article

130 Citations (Scopus)

Abstract

The transcription factor PU.1 is an important regulator of hematopoiesis; precise expression levels are critical for normal hematopoietic development and suppression of leukemia. We show here that noncoding antisense RNAs are important modulators of proper dosages of PU.1. Antisense and sense RNAs are regulated by shared evolutionarily conserved cis-regulatory elements, and we can show that antisense RNAs inhibit PU.1 expression by modulating mRNA translation. We propose that such antisense RNAs will likely be important in the regulation of many genes and may be the reason for the large number of overlapping complementary transcripts with so far unknown function.

Original language	English
Pages (from-to)	2085-2092
Number of pages	8
Journal	Genes & Development
Volume	22
Issue number	15
DOIs	https://doi.org/10.1101/gad.1654808
Publication status	Published - 1 Aug 2008

Keywords

translation stalling
upstream and intronic regulatory elements
coordinated expression of the target and regulator
noncoding antisense RNA

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1101/gad.1654808

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Ebralidze, AK., Guibal, FC., Steidl, U., Zhang, P., Lee, S., Bartholdy, B., Jorda, MA., Petkova, V., Rosenbauer, F., Huang, G., Dayaram, T., Klupp, J., O'Brien, KB., Will, B., Hoogenkamp, M., Borden, KLB., Bonifer, C., & Tenen, DG. (2008). PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element. Genes & Development, 22(15), 2085-2092. https://doi.org/10.1101/gad.1654808

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title = "PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element",

abstract = "The transcription factor PU.1 is an important regulator of hematopoiesis; precise expression levels are critical for normal hematopoietic development and suppression of leukemia. We show here that noncoding antisense RNAs are important modulators of proper dosages of PU.1. Antisense and sense RNAs are regulated by shared evolutionarily conserved cis-regulatory elements, and we can show that antisense RNAs inhibit PU.1 expression by modulating mRNA translation. We propose that such antisense RNAs will likely be important in the regulation of many genes and may be the reason for the large number of overlapping complementary transcripts with so far unknown function.",

keywords = "translation stalling, upstream and intronic regulatory elements, coordinated expression of the target and regulator, noncoding antisense RNA",

author = "AK Ebralidze and FC Guibal and U Steidl and P Zhang and S Lee and B Bartholdy and MA Jorda and V Petkova and F Rosenbauer and G Huang and T Dayaram and J Klupp and KB O'Brien and B Will and Maarten Hoogenkamp and KLB Borden and Constanze Bonifer and DG Tenen",

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doi = "10.1101/gad.1654808",

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Ebralidze, AK, Guibal, FC, Steidl, U, Zhang, P, Lee, S, Bartholdy, B, Jorda, MA, Petkova, V, Rosenbauer, F, Huang, G, Dayaram, T, Klupp, J, O'Brien, KB, Will, B, Hoogenkamp, M, Borden, KLB, Bonifer, C & Tenen, DG 2008, 'PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element', Genes & Development, vol. 22, no. 15, pp. 2085-2092. https://doi.org/10.1101/gad.1654808

TY - JOUR

T1 - PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element

AU - Ebralidze, AK

AU - Guibal, FC

AU - Steidl, U

AU - Zhang, P

AU - Lee, S

AU - Bartholdy, B

AU - Jorda, MA

AU - Petkova, V

AU - Rosenbauer, F

AU - Huang, G

AU - Dayaram, T

AU - Klupp, J

AU - O'Brien, KB

AU - Will, B

AU - Hoogenkamp, Maarten

AU - Borden, KLB

AU - Bonifer, Constanze

AU - Tenen, DG

PY - 2008/8/1

Y1 - 2008/8/1

N2 - The transcription factor PU.1 is an important regulator of hematopoiesis; precise expression levels are critical for normal hematopoietic development and suppression of leukemia. We show here that noncoding antisense RNAs are important modulators of proper dosages of PU.1. Antisense and sense RNAs are regulated by shared evolutionarily conserved cis-regulatory elements, and we can show that antisense RNAs inhibit PU.1 expression by modulating mRNA translation. We propose that such antisense RNAs will likely be important in the regulation of many genes and may be the reason for the large number of overlapping complementary transcripts with so far unknown function.

AB - The transcription factor PU.1 is an important regulator of hematopoiesis; precise expression levels are critical for normal hematopoietic development and suppression of leukemia. We show here that noncoding antisense RNAs are important modulators of proper dosages of PU.1. Antisense and sense RNAs are regulated by shared evolutionarily conserved cis-regulatory elements, and we can show that antisense RNAs inhibit PU.1 expression by modulating mRNA translation. We propose that such antisense RNAs will likely be important in the regulation of many genes and may be the reason for the large number of overlapping complementary transcripts with so far unknown function.

KW - translation stalling

KW - upstream and intronic regulatory elements

KW - coordinated expression of the target and regulator

KW - noncoding antisense RNA

U2 - 10.1101/gad.1654808

DO - 10.1101/gad.1654808

M3 - Article

C2 - 18676813

SN - 0890-9369

VL - 22

SP - 2085

EP - 2092

JO - Genes & Development

JF - Genes & Development

IS - 15

ER -

PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element

Abstract

Keywords

ASJC Scopus subject areas

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