Protocol for intelligent high-content screening of zebrafish embryos on a standard widefield screening microscope

Research output: Contribution to journalArticle

Authors

  • Ravindra Peravali
  • Jochen Gehrig
  • Stefan Giselbrecht
  • Dominic Lütjohann
  • Urban Liebel

External organisations

  • Karlsruhe Institute of Technology, Campus North
  • Department of Medical and Molecular Genetics

Abstract

Here we demonstrate a toolset for automated intelligent high-content screening of whole zebrafish embryos at cellular resolution on a standard wide-field screening microscope. Using custom-developed algorithms, predefined regions of interest, such as the brain, are automatically detected. The regions of interest are subsequently imaged automatically at high magnification, enabling rapid capture of cellular resolution data. standardized imaging of internal organs and cellular structures.

Details

Original languageEnglish
Pages (from-to)xx
Number of pages1
JournalBioTechniques
Volume62
Issue number1
Publication statusPublished - Jan 2017