Protocol for intelligent high-content screening of zebrafish embryos on a standard widefield screening microscope

Research output: Other contribution

Authors

  • Ravindra Peravali
  • Jochen Gehrig
  • Stefan Giselbrecht
  • Dominic Lütjohann
  • Urban Liebel

External organisations

  • Karlsruhe Institute of Technology, Campus North

Abstract

Here we demonstrate a toolset for automated intelligent high-content screening of whole zebrafish embryos at cellular resolution on a standard wide-field screening microscope. Using custom-developed algorithms, predefined regions of interest, such as the brain, are automatically detected. The regions of interest are subsequently imaged automatically at high magnification, enabling rapid capture of cellular resolution data. standardized imaging of internal organs and cellular structures.

Details

Original languageEnglish
Number of pages1
Edition1
Volume62
Publication statusPublished - 31 Jan 2017

Publication series

NameBioTechniques
PublisherEaton Publishing Company
ISSN (Print)0736-6205