Protocol for assay of transposase accessible chromatin sequencing in non-model species

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Colleges, School and Institutes


The assay for transposase accessible chromatin (ATAC-seq) is a method for mapping genome-wide chromatin accessibility. Coupled with high-throughput sequencing, it enables integrative epigenomics analyses. ATAC-seq requires direct access to cell nuclei, a major challenge in non-model species such as small invertebrates, whose soft tissue is surrounded by a protective exoskeleton. Here, we present modifications of the ATAC-seq protocol for applications in small crustaceans, extending applications to non-model species.

For complete information on the use and execution of this protocol, please refer to Buenrostro et al. (2013).


Original languageEnglish
JournalSTAR Protocols
Early online date13 Feb 2021
Publication statusPublished - 19 Mar 2021