Protocol for assay of transposase accessible chromatin sequencing in non-model species

Research output: Contribution to journalArticlepeer-review

Authors

Colleges, School and Institutes

Abstract

The assay for transposase accessible chromatin (ATAC-seq) is a method for mapping genome-wide chromatin accessibility. Coupled with high-throughput sequencing, it enables integrative epigenomics analyses. ATAC-seq requires direct access to cell nuclei, a major challenge in non-model species such as small invertebrates, whose soft tissue is surrounded by a protective exoskeleton. Here, we present modifications of the ATAC-seq protocol for applications in small crustaceans, extending applications to non-model species.

For complete information on the use and execution of this protocol, please refer to Buenrostro et al. (2013).

Details

Original languageEnglish
JournalSTAR Protocols
Volumehttps://doi.org/10.1016/j.xpro.2021.100341
Early online date13 Feb 2021
Publication statusPublished - 19 Mar 2021