Programmed assembly of polymer-DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition

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Programmed assembly of polymer-DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition. / Magnusson, J.P.; Fernández-Trillo, F.; Sicilia, G.; Spain, S.G.; Alexander, C.

In: Nanoscale, Vol. 6, No. 4, 21.02.2014, p. 2368-2374.

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@article{f4e3f40c612e4372ac6efc34375a638d,
title = "Programmed assembly of polymer-DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition",
abstract = "Soft micellar nanoparticles can be prepared from DNA conjugates designed to assemble via base pairing such that strands containing a polymer corona and a cholesterol tail generate controlled supramolecular architecture. Functionalization of one DNA conjugate strand with a biorecognition ligand results in shielding of the ligand when in the micelle, while encoding of the DNA sequences with overhangs allows supramolecular unpacking by addition of a complementary strand and sequence-specific unshielding of the ligand. The molecular assembly/disassembly and 'on-off' switch of the recognition signal is visualized by FRET pair signalling, PAGE and a facile turbidimetric binding assay, allowing direct and amplified readout of nucleic acid sequence recognition. {\textcopyright} 2014 The Royal Society of Chemistry.",
author = "J.P. Magnusson and F. Fern{\'a}ndez-Trillo and G. Sicilia and S.G. Spain and C. Alexander",
year = "2014",
month = feb,
day = "21",
doi = "10.1039/c3nr04952c",
language = "English",
volume = "6",
pages = "2368--2374",
journal = "Nanoscale",
issn = "2040-3364",
publisher = "Royal Society of Chemistry",
number = "4",

}

RIS

TY - JOUR

T1 - Programmed assembly of polymer-DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition

AU - Magnusson, J.P.

AU - Fernández-Trillo, F.

AU - Sicilia, G.

AU - Spain, S.G.

AU - Alexander, C.

PY - 2014/2/21

Y1 - 2014/2/21

N2 - Soft micellar nanoparticles can be prepared from DNA conjugates designed to assemble via base pairing such that strands containing a polymer corona and a cholesterol tail generate controlled supramolecular architecture. Functionalization of one DNA conjugate strand with a biorecognition ligand results in shielding of the ligand when in the micelle, while encoding of the DNA sequences with overhangs allows supramolecular unpacking by addition of a complementary strand and sequence-specific unshielding of the ligand. The molecular assembly/disassembly and 'on-off' switch of the recognition signal is visualized by FRET pair signalling, PAGE and a facile turbidimetric binding assay, allowing direct and amplified readout of nucleic acid sequence recognition. © 2014 The Royal Society of Chemistry.

AB - Soft micellar nanoparticles can be prepared from DNA conjugates designed to assemble via base pairing such that strands containing a polymer corona and a cholesterol tail generate controlled supramolecular architecture. Functionalization of one DNA conjugate strand with a biorecognition ligand results in shielding of the ligand when in the micelle, while encoding of the DNA sequences with overhangs allows supramolecular unpacking by addition of a complementary strand and sequence-specific unshielding of the ligand. The molecular assembly/disassembly and 'on-off' switch of the recognition signal is visualized by FRET pair signalling, PAGE and a facile turbidimetric binding assay, allowing direct and amplified readout of nucleic acid sequence recognition. © 2014 The Royal Society of Chemistry.

UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84893327896&partnerID=8YFLogxK

U2 - 10.1039/c3nr04952c

DO - 10.1039/c3nr04952c

M3 - Article

C2 - 24271079

VL - 6

SP - 2368

EP - 2374

JO - Nanoscale

JF - Nanoscale

SN - 2040-3364

IS - 4

ER -