Production of membrane proteins without cells or detergents

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Production of membrane proteins without cells or detergents. / Rajesh, Sundaresan; Knowles, Timothy; Overduin, Michael.

In: New Biotechnology, Vol. 28, No. 3, 30.04.2011, p. 250-254.

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@article{e21719c81d724c44a1fb14f810e1e29e,
title = "Production of membrane proteins without cells or detergents",
abstract = "The production of membrane proteins in cellular systems is besieged by several problems due to their hydrophobic nature which often causes misfolding, protein aggregation and cytotoxicity, resulting in poor yields of stable proteins. Cell-free expression has emerged as one of the most versatile alternatives for circumventing these obstacles by producing membrane proteins directly into designed hydrophobic environments. Efficient optimisation of expression and solubilisation conditions using a variety of detergents, membrane mimetics and lipids has yielded structurally and functionally intact membrane proteins, with yields several fold above the levels possible from cell-based systems. Here we review recently developed techniques available to produce functional membrane proteins, and discuss amphipols, nanodisc and styrene maleic acid lipid particle (SMALP) technologies that can be exploited alongside cell-free expression of membrane proteins.",
author = "Sundaresan Rajesh and Timothy Knowles and Michael Overduin",
year = "2011",
month = apr,
day = "30",
doi = "10.1016/j.nbt.2010.07.011",
language = "English",
volume = "28",
pages = "250--254",
journal = "New Biotechnology",
issn = "1871-6784",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Production of membrane proteins without cells or detergents

AU - Rajesh, Sundaresan

AU - Knowles, Timothy

AU - Overduin, Michael

PY - 2011/4/30

Y1 - 2011/4/30

N2 - The production of membrane proteins in cellular systems is besieged by several problems due to their hydrophobic nature which often causes misfolding, protein aggregation and cytotoxicity, resulting in poor yields of stable proteins. Cell-free expression has emerged as one of the most versatile alternatives for circumventing these obstacles by producing membrane proteins directly into designed hydrophobic environments. Efficient optimisation of expression and solubilisation conditions using a variety of detergents, membrane mimetics and lipids has yielded structurally and functionally intact membrane proteins, with yields several fold above the levels possible from cell-based systems. Here we review recently developed techniques available to produce functional membrane proteins, and discuss amphipols, nanodisc and styrene maleic acid lipid particle (SMALP) technologies that can be exploited alongside cell-free expression of membrane proteins.

AB - The production of membrane proteins in cellular systems is besieged by several problems due to their hydrophobic nature which often causes misfolding, protein aggregation and cytotoxicity, resulting in poor yields of stable proteins. Cell-free expression has emerged as one of the most versatile alternatives for circumventing these obstacles by producing membrane proteins directly into designed hydrophobic environments. Efficient optimisation of expression and solubilisation conditions using a variety of detergents, membrane mimetics and lipids has yielded structurally and functionally intact membrane proteins, with yields several fold above the levels possible from cell-based systems. Here we review recently developed techniques available to produce functional membrane proteins, and discuss amphipols, nanodisc and styrene maleic acid lipid particle (SMALP) technologies that can be exploited alongside cell-free expression of membrane proteins.

U2 - 10.1016/j.nbt.2010.07.011

DO - 10.1016/j.nbt.2010.07.011

M3 - Article

C2 - 20654746

VL - 28

SP - 250

EP - 254

JO - New Biotechnology

JF - New Biotechnology

SN - 1871-6784

IS - 3

ER -