Production of 1,2-diacylglycerol in human erythrocyte membranes exposed to low concentrations of calcium ions

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Production of 1,2-diacylglycerol in human erythrocyte membranes exposed to low concentrations of calcium ions. / Allan, David; Michell, Robert H.

In: BBA - Biomembranes, Vol. 455, No. 3, 14.12.1976, p. 824-830.

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@article{eda56c0f059f4b53bc79b81fa768233c,
title = "Production of 1,2-diacylglycerol in human erythrocyte membranes exposed to low concentrations of calcium ions",
abstract = "A specific increase in the membrane content of 1,2-diacylglycerol occurred when erythorcytes were lysed at 20 °C in media which did not include a chelator of Ca2+ and also when Ca2+ was added to haemoglobin-free erythrocyte ghosts which had been prepared in the presence of ethyleneglycol-bis-(β-aminoethylether)-N,N′-tetraacetic acid (EGTA). The maximum increase was about 20-fold. The production of 1,2-diacylglycerol appeared to be caused by an endogenous membrane-bound phospholipase C which was half-maximally activated at less than 1 μM Ca2+ and which had access to only about 0.6-0.8% of the cells' glycerolipids. This activity was optimal at pH 7.0-7.2 in the presence of 0.1 mM Ca2+; under these conditions diacylglycerol production was complete within 5-10 min. Enzyme activity was markedly decreased at low temperatures, and was abolished by heating at 100 °C for 1 min.",
author = "David Allan and Michell, {Robert H.}",
year = "1976",
month = dec,
day = "14",
doi = "10.1016/0005-2736(76)90052-3",
language = "English",
volume = "455",
pages = "824--830",
journal = "Biochimica et Biophysica Acta (BBA) - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Production of 1,2-diacylglycerol in human erythrocyte membranes exposed to low concentrations of calcium ions

AU - Allan, David

AU - Michell, Robert H.

PY - 1976/12/14

Y1 - 1976/12/14

N2 - A specific increase in the membrane content of 1,2-diacylglycerol occurred when erythorcytes were lysed at 20 °C in media which did not include a chelator of Ca2+ and also when Ca2+ was added to haemoglobin-free erythrocyte ghosts which had been prepared in the presence of ethyleneglycol-bis-(β-aminoethylether)-N,N′-tetraacetic acid (EGTA). The maximum increase was about 20-fold. The production of 1,2-diacylglycerol appeared to be caused by an endogenous membrane-bound phospholipase C which was half-maximally activated at less than 1 μM Ca2+ and which had access to only about 0.6-0.8% of the cells' glycerolipids. This activity was optimal at pH 7.0-7.2 in the presence of 0.1 mM Ca2+; under these conditions diacylglycerol production was complete within 5-10 min. Enzyme activity was markedly decreased at low temperatures, and was abolished by heating at 100 °C for 1 min.

AB - A specific increase in the membrane content of 1,2-diacylglycerol occurred when erythorcytes were lysed at 20 °C in media which did not include a chelator of Ca2+ and also when Ca2+ was added to haemoglobin-free erythrocyte ghosts which had been prepared in the presence of ethyleneglycol-bis-(β-aminoethylether)-N,N′-tetraacetic acid (EGTA). The maximum increase was about 20-fold. The production of 1,2-diacylglycerol appeared to be caused by an endogenous membrane-bound phospholipase C which was half-maximally activated at less than 1 μM Ca2+ and which had access to only about 0.6-0.8% of the cells' glycerolipids. This activity was optimal at pH 7.0-7.2 in the presence of 0.1 mM Ca2+; under these conditions diacylglycerol production was complete within 5-10 min. Enzyme activity was markedly decreased at low temperatures, and was abolished by heating at 100 °C for 1 min.

UR - http://www.scopus.com/inward/record.url?scp=0017177547&partnerID=8YFLogxK

U2 - 10.1016/0005-2736(76)90052-3

DO - 10.1016/0005-2736(76)90052-3

M3 - Article

C2 - 826280

AN - SCOPUS:0017177547

VL - 455

SP - 824

EP - 830

JO - Biochimica et Biophysica Acta (BBA) - Biomembranes

JF - Biochimica et Biophysica Acta (BBA) - Biomembranes

SN - 0005-2736

IS - 3

ER -