Probing cancer cell metabolism using NMR spectroscopy

Research output: Chapter in Book/Report/Conference proceedingChapter

Authors

External organisations

  • Institute of Metabolism and Systems Research

Abstract

Altered cellular metabolism is now accepted to be at the core of many diseases including cancer. Over the past 20 years, NMR has become a core technology to study these metabolic perturbations in detail. This chapter reviews current NMR-based methods for steady-state metabolism and, in particular, the use of non-radioactive stable isotope-enriched tracers. Opportunities and challenges for each method, such as 1D (1)H NMR spectroscopy and (13)C carbon-based NMR spectroscopic methods, are discussed. Ultimately, the combination of NMR and mass spectra as orthogonal technologies are required to compensate for the drawbacks of each technique when used singly are discussed.

Details

Original languageEnglish
Title of host publicationTumor Microenvironment
Subtitle of host publicationStudy Protocols
Publication statusPublished - 2016
Event3rd International Conference on Tumor Microenvironment and Cellular Stress - Mykonos, Greece
Duration: 21 Sep 201426 Sep 2014

Publication series

NameAdvances in Experimental Medicine and Biology
Volume899
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Conference

Conference3rd International Conference on Tumor Microenvironment and Cellular Stress
CountryGreece
CityMykonos
Period21/09/1426/09/14

Keywords

  • 13C, 1H, Cancer, HSQC, Metabolism, Metabolomics, MS, NMR, Stable isotopes, Tracer analysis