Primary capture of high molecular weight nucleic acids using aqueous two-phase systems
Research output: Contribution to journal › Article
Colleges, School and Institutes
The practical feasibility and generic applicability of the selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol 300 (PEG-300) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) by addition of NaCl salt was investigated with partially disrupted Escherichia coli (E. coli) cell paste. The process yielded a relatively protein-free pDNA solution with 89.5% pDNA recovery while low molecular weight RNA was partially removed. The results gained here led the way to an alternative process options for the processing of pDNA. A reproducible process involving two extraction steps to recover pDNA from partially disrupted E. coli cell paste was developed and is presented here. (C) 2008 Elsevier B.V. All rights reserved.
|Number of pages||6|
|Journal||Separation and Purification Technology|
|Publication status||Published - 1 Apr 2009|
- ATPS, Selective partition, Plasmid DNA, NaCl, E. coli cell paste