Prevention of coronal discoloration induced by regenerative endodontic treatment in an ex vivo model

Research output: Contribution to journalArticle


  • Noushin Shokouhinejad
  • Mehrfam Khoshkhounejad
  • Marzieh Alikhasi
  • Parisa Bagheri
  • Josette Camilleri

Colleges, School and Institutes


Objectives: The aim of this study was to assess the effect of sealing the pulp chamber walls with a dentin-bonding agent (DBA) on prevention of discoloration induced by regenerative endodontic procedures (REPs) in an ex vivo model.Materials and methods: Ninety-six bovine incisors were prepared and randomly divided into two groups. In one group, the pulp chamber walls were sealed with DBA before placement of triple antibiotic paste (TAP) containing minocycline inside the root canals, but in the other group, DBA was not applied. After 4 weeks, the root canals were filled with human blood and each group was then randomly divided into four subgroups (n = 12) according to the endodontic cements placed over the blood clot (ProRoot MTA, OrthoMTA, RetroMTA, or Biodentine). The color changes (∆E) were measured at different steps. The data were analyzed using t test and two-way ANOVA.ResultsThe specimens in which dentinal walls of pulp chamber were sealed with DBA showed significantly less coronal discoloration at each step of regenerative treatment (p < 0.001). However, application of DBA did not completely prevent the clinically perceptible coronal color change. Sealing the blood clot with different endodontic cements did not result in significant difference in coronal discoloration (p > 0.05).ConclusionsSealing the pulp chamber walls before insertion of TAP decreased coronal discoloration following REP using different endodontic cements but did not prevent it.


Original languageEnglish
Pages (from-to)1725–1731
Number of pages7
JournalClinical Oral Investigations
Issue number4
Early online date31 Oct 2017
Publication statusPublished - May 2018