Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks

Johanna M C Jefferies; Calum H G Johnston; Lea-Ann S Kirkham; Graeme J M Cowan; Kirsty S Ross; Andrew Smith; Stuart C Clarke; Angela B Brueggemann; Robert C George; Bruno Pichon; Gerd Pluschke; Valentin Pfluger; Tim J Mitchell

doi:10.1086/520091

Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks

Johanna M C Jefferies, Calum H G Johnston, Lea-Ann S Kirkham, Graeme J M Cowan, Kirsty S Ross, Andrew Smith, Stuart C Clarke, Angela B Brueggemann, Robert C George, Bruno Pichon, Gerd Pluschke, Valentin Pfluger, Tim J Mitchell

Immunology and Immunotherapy

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

Pneumolysin is an important virulence factor of the human pathogen Streptococcus pneumoniae. Sequence analysis of the ply gene from 121 clinical isolates of S. pneumoniae uncovered a number of alleles. Twenty-two strains were chosen for further analysis, and 14 protein alleles were discovered. Five of these had been reported previously, and the remaining 9 were novel. Cell lysates were used to determine the specific hemolytic activities of the pneumolysin proteins. Six strains showed no hemolytic activity, and the remaining 16 were hemolytic, to varying degrees. We report that the nonhemolytic allele reported previously in serotype 1, sequence type (ST) 306 isolates is also present in a number of pneumococcal isolates of serotype 8 that belong to the ST53 lineage. Serotype 1 and 8 pneumococci are known to be associated with outbreaks of invasive disease. The nonhemolytic pneumolysin allele is therefore associated with the dominant clones of outbreak-associated serotypes of S. pneumoniae.

Original language	English
Pages (from-to)	936-44
Number of pages	9
Journal	The Journal of Infectious Diseases
Volume	196
Issue number	6
DOIs	https://doi.org/10.1086/520091
Publication status	Published - 15 Sept 2007

Keywords

Alleles
Bacterial Proteins
Cluster Analysis
DNA, Bacterial
Disease Outbreaks
Genotype
Hemolysis
Humans
Molecular Sequence Data
Pneumococcal Infections
Polymorphism, Genetic
Sequence Analysis, DNA
Sequence Homology
Serotyping
Streptococcus pneumoniae
Streptolysins
Virulence Factors

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1086/520091

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Jefferies, J. M. C., Johnston, C. H. G., Kirkham, L-A. S., Cowan, G. J. M., Ross, K. S., Smith, A., Clarke, S. C., Brueggemann, A. B., George, R. C., Pichon, B., Pluschke, G., Pfluger, V., & Mitchell, T. J. (2007). Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks. The Journal of Infectious Diseases, 196(6), 936-44. https://doi.org/10.1086/520091

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title = "Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks",

abstract = "Pneumolysin is an important virulence factor of the human pathogen Streptococcus pneumoniae. Sequence analysis of the ply gene from 121 clinical isolates of S. pneumoniae uncovered a number of alleles. Twenty-two strains were chosen for further analysis, and 14 protein alleles were discovered. Five of these had been reported previously, and the remaining 9 were novel. Cell lysates were used to determine the specific hemolytic activities of the pneumolysin proteins. Six strains showed no hemolytic activity, and the remaining 16 were hemolytic, to varying degrees. We report that the nonhemolytic allele reported previously in serotype 1, sequence type (ST) 306 isolates is also present in a number of pneumococcal isolates of serotype 8 that belong to the ST53 lineage. Serotype 1 and 8 pneumococci are known to be associated with outbreaks of invasive disease. The nonhemolytic pneumolysin allele is therefore associated with the dominant clones of outbreak-associated serotypes of S. pneumoniae.",

keywords = "Alleles, Bacterial Proteins, Cluster Analysis, DNA, Bacterial, Disease Outbreaks, Genotype, Hemolysis, Humans, Molecular Sequence Data, Pneumococcal Infections, Polymorphism, Genetic, Sequence Analysis, DNA, Sequence Homology, Serotyping, Streptococcus pneumoniae, Streptolysins, Virulence Factors",

author = "Jefferies, {Johanna M C} and Johnston, {Calum H G} and Kirkham, {Lea-Ann S} and Cowan, {Graeme J M} and Ross, {Kirsty S} and Andrew Smith and Clarke, {Stuart C} and Brueggemann, {Angela B} and George, {Robert C} and Bruno Pichon and Gerd Pluschke and Valentin Pfluger and Mitchell, {Tim J}",

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Jefferies, JMC, Johnston, CHG, Kirkham, L-AS, Cowan, GJM, Ross, KS, Smith, A, Clarke, SC, Brueggemann, AB, George, RC, Pichon, B, Pluschke, G, Pfluger, V & Mitchell, TJ 2007, 'Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks', The Journal of Infectious Diseases, vol. 196, no. 6, pp. 936-44. https://doi.org/10.1086/520091

TY - JOUR

T1 - Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks

AU - Jefferies, Johanna M C

AU - Johnston, Calum H G

AU - Kirkham, Lea-Ann S

AU - Cowan, Graeme J M

AU - Ross, Kirsty S

AU - Smith, Andrew

AU - Clarke, Stuart C

AU - Brueggemann, Angela B

AU - George, Robert C

AU - Pichon, Bruno

AU - Pluschke, Gerd

AU - Pfluger, Valentin

AU - Mitchell, Tim J

PY - 2007/9/15

Y1 - 2007/9/15

N2 - Pneumolysin is an important virulence factor of the human pathogen Streptococcus pneumoniae. Sequence analysis of the ply gene from 121 clinical isolates of S. pneumoniae uncovered a number of alleles. Twenty-two strains were chosen for further analysis, and 14 protein alleles were discovered. Five of these had been reported previously, and the remaining 9 were novel. Cell lysates were used to determine the specific hemolytic activities of the pneumolysin proteins. Six strains showed no hemolytic activity, and the remaining 16 were hemolytic, to varying degrees. We report that the nonhemolytic allele reported previously in serotype 1, sequence type (ST) 306 isolates is also present in a number of pneumococcal isolates of serotype 8 that belong to the ST53 lineage. Serotype 1 and 8 pneumococci are known to be associated with outbreaks of invasive disease. The nonhemolytic pneumolysin allele is therefore associated with the dominant clones of outbreak-associated serotypes of S. pneumoniae.

AB - Pneumolysin is an important virulence factor of the human pathogen Streptococcus pneumoniae. Sequence analysis of the ply gene from 121 clinical isolates of S. pneumoniae uncovered a number of alleles. Twenty-two strains were chosen for further analysis, and 14 protein alleles were discovered. Five of these had been reported previously, and the remaining 9 were novel. Cell lysates were used to determine the specific hemolytic activities of the pneumolysin proteins. Six strains showed no hemolytic activity, and the remaining 16 were hemolytic, to varying degrees. We report that the nonhemolytic allele reported previously in serotype 1, sequence type (ST) 306 isolates is also present in a number of pneumococcal isolates of serotype 8 that belong to the ST53 lineage. Serotype 1 and 8 pneumococci are known to be associated with outbreaks of invasive disease. The nonhemolytic pneumolysin allele is therefore associated with the dominant clones of outbreak-associated serotypes of S. pneumoniae.

KW - Alleles

KW - Bacterial Proteins

KW - Cluster Analysis

KW - DNA, Bacterial

KW - Disease Outbreaks

KW - Genotype

KW - Hemolysis

KW - Humans

KW - Molecular Sequence Data

KW - Pneumococcal Infections

KW - Polymorphism, Genetic

KW - Sequence Analysis, DNA

KW - Sequence Homology

KW - Serotyping

KW - Streptococcus pneumoniae

KW - Streptolysins

KW - Virulence Factors

U2 - 10.1086/520091

DO - 10.1086/520091

M3 - Article

C2 - 17703426

SN - 0022-1899

VL - 196

SP - 936

EP - 944

JO - The Journal of Infectious Diseases

JF - The Journal of Infectious Diseases

IS - 6

ER -

Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks

Abstract

Keywords

UN SDGs

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