Preprocessing, analysis and quantification in single‐voxel magnetic resonance spectroscopy: experts' consensus recommendations

Research output: Contribution to journalReview articlepeer-review


  • Jamie Near
  • Ashley D. Harris
  • Christoph Juchem
  • Roland Kreis
  • Małgorzata Marjańska
  • Gülin Öz
  • Johannes Slotboom
  • Charles Gasparovic


Once an MRS dataset has been acquired, several important steps must be taken to obtain the desired metabolite concentration measures. First, the data must be preprocessed to prepare them for analysis. Next, the intensity of the metabolite signal(s) of interest must be estimated. Finally, the measured metabolite signal intensities must be converted into scaled concentration units employing a quantitative reference signal to allow meaningful interpretation. In this paper, we review these three main steps in the post‐acquisition workflow of a single‐voxel MRS experiment (preprocessing, analysis and quantification) and provide recommendations for best practices at each step.


Original languageEnglish
Article numbere4257
Number of pages23
JournalNMR in biomedicine
Early online date21 Feb 2020
Publication statusE-pub ahead of print - 21 Feb 2020


  • analysis, MRS, preprocessing, processing, quantification, quantitation, water referencing