Preparation of erythrocyte ghosts by a glycol-induced osmotic lysis under isoionic conditions

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Preparation of erythrocyte ghosts by a glycol-induced osmotic lysis under isoionic conditions. / Billah, M. M.; Finean, J. B.; Coleman, R.; Michell, R. H.

In: BBA - Biomembranes, Vol. 433, No. 1, 16.04.1976, p. 54-62.

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@article{ba402d014dea456788dd3443d2928fd6,
title = "Preparation of erythrocyte ghosts by a glycol-induced osmotic lysis under isoionic conditions",
abstract = "A procedure has been developed for obtaining haemoglobin-free, erythrocyte ghosts under ionic conditions approximating that of the cell cytoplasm. Haemolysis was effected by incorporating glycol into cells suspended in the isoionic medium and then diluting with a large volume of glycol-free medium. The ghosts were of uniform spherical shape throughout the preparative procedure and were impermeable to macromolecules. Analysis of polypeptides by sodium dodecyl sulphate-gel elecrophoresis at each stage of preparation and comparison with ghosts prepared under hypo-ionic conditions served to distinguish membrane components from those of cytoplasm.",
author = "Billah, {M. M.} and Finean, {J. B.} and R. Coleman and Michell, {R. H.}",
year = "1976",
month = apr,
day = "16",
doi = "10.1016/0005-2736(76)90177-2",
language = "English",
volume = "433",
pages = "54--62",
journal = "Biochimica et Biophysica Acta (BBA) - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Preparation of erythrocyte ghosts by a glycol-induced osmotic lysis under isoionic conditions

AU - Billah, M. M.

AU - Finean, J. B.

AU - Coleman, R.

AU - Michell, R. H.

PY - 1976/4/16

Y1 - 1976/4/16

N2 - A procedure has been developed for obtaining haemoglobin-free, erythrocyte ghosts under ionic conditions approximating that of the cell cytoplasm. Haemolysis was effected by incorporating glycol into cells suspended in the isoionic medium and then diluting with a large volume of glycol-free medium. The ghosts were of uniform spherical shape throughout the preparative procedure and were impermeable to macromolecules. Analysis of polypeptides by sodium dodecyl sulphate-gel elecrophoresis at each stage of preparation and comparison with ghosts prepared under hypo-ionic conditions served to distinguish membrane components from those of cytoplasm.

AB - A procedure has been developed for obtaining haemoglobin-free, erythrocyte ghosts under ionic conditions approximating that of the cell cytoplasm. Haemolysis was effected by incorporating glycol into cells suspended in the isoionic medium and then diluting with a large volume of glycol-free medium. The ghosts were of uniform spherical shape throughout the preparative procedure and were impermeable to macromolecules. Analysis of polypeptides by sodium dodecyl sulphate-gel elecrophoresis at each stage of preparation and comparison with ghosts prepared under hypo-ionic conditions served to distinguish membrane components from those of cytoplasm.

UR - http://www.scopus.com/inward/record.url?scp=0017249667&partnerID=8YFLogxK

U2 - 10.1016/0005-2736(76)90177-2

DO - 10.1016/0005-2736(76)90177-2

M3 - Article

C2 - 769836

AN - SCOPUS:0017249667

VL - 433

SP - 54

EP - 62

JO - Biochimica et Biophysica Acta (BBA) - Biomembranes

JF - Biochimica et Biophysica Acta (BBA) - Biomembranes

SN - 0005-2736

IS - 1

ER -