Preparation of erythrocyte ghosts by a glycol-induced osmotic lysis under isoionic conditions

Research output: Contribution to journalArticlepeer-review

Authors

Colleges, School and Institutes

External organisations

  • Birmingham University
  • Department of Biochemistry

Abstract

A procedure has been developed for obtaining haemoglobin-free, erythrocyte ghosts under ionic conditions approximating that of the cell cytoplasm. Haemolysis was effected by incorporating glycol into cells suspended in the isoionic medium and then diluting with a large volume of glycol-free medium. The ghosts were of uniform spherical shape throughout the preparative procedure and were impermeable to macromolecules. Analysis of polypeptides by sodium dodecyl sulphate-gel elecrophoresis at each stage of preparation and comparison with ghosts prepared under hypo-ionic conditions served to distinguish membrane components from those of cytoplasm.

Details

Original languageEnglish
Pages (from-to)54-62
Number of pages9
JournalBBA - Biomembranes
Volume433
Issue number1
Publication statusPublished - 16 Apr 1976