Preparation and antimicrobial evaluation of polyion complex (PIC) nanoparticles loaded with polymyxin B

Research output: Contribution to journalArticle

Standard

Harvard

APA

Vancouver

Author

Bibtex

@article{71231f92f15744f1b7fede85fb0cf182,
title = "Preparation and antimicrobial evaluation of polyion complex (PIC) nanoparticles loaded with polymyxin B",
abstract = "Here, we describe novel polyion complex (PIC) particles for the delivery of Polymyxin B (Pol-B), an antimicrobial peptide currently used in the clinic as a last resort antibiotic against multidrug-resistant gram-negative bacteria. A range of conditions for the controlled assembly of Pol-B with poly(styrene sulphonate) (PSS) has been identified which let us prepare stable colloidal PIC particles. This way, PIC particles containing different Pol-B:PSS ratios have been prepared and their stability under simulated physiological conditions (i.e. pH, osmotic pressure and temperature) characterised. Furthermore, preliminary evaluation of the antimicrobial activity of these Pol-B containing PIC particles has been performed, by monitoring their effect on the growth of Pseudomonas aeruginosa, an opportunistic gram-negative bacterium.",
keywords = "PIC particles , Polyelectrolyte complexes , Antimicrobials , Drug delivery",
author = "{Insua Lopez}, Ignacio and Sieta Majok and Peacock, {Anna F.a.} and {Marie Krachler}, Anne and Francisco Fernandez-trillo",
year = "2017",
month = feb,
doi = "10.1016/j.eurpolymj.2016.08.023",
language = "English",
volume = "87",
pages = "478–486",
journal = "European Polymer Journal",
issn = "0014-3057",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Preparation and antimicrobial evaluation of polyion complex (PIC) nanoparticles loaded with polymyxin B

AU - Insua Lopez, Ignacio

AU - Majok, Sieta

AU - Peacock, Anna F.a.

AU - Marie Krachler, Anne

AU - Fernandez-trillo, Francisco

PY - 2017/2

Y1 - 2017/2

N2 - Here, we describe novel polyion complex (PIC) particles for the delivery of Polymyxin B (Pol-B), an antimicrobial peptide currently used in the clinic as a last resort antibiotic against multidrug-resistant gram-negative bacteria. A range of conditions for the controlled assembly of Pol-B with poly(styrene sulphonate) (PSS) has been identified which let us prepare stable colloidal PIC particles. This way, PIC particles containing different Pol-B:PSS ratios have been prepared and their stability under simulated physiological conditions (i.e. pH, osmotic pressure and temperature) characterised. Furthermore, preliminary evaluation of the antimicrobial activity of these Pol-B containing PIC particles has been performed, by monitoring their effect on the growth of Pseudomonas aeruginosa, an opportunistic gram-negative bacterium.

AB - Here, we describe novel polyion complex (PIC) particles for the delivery of Polymyxin B (Pol-B), an antimicrobial peptide currently used in the clinic as a last resort antibiotic against multidrug-resistant gram-negative bacteria. A range of conditions for the controlled assembly of Pol-B with poly(styrene sulphonate) (PSS) has been identified which let us prepare stable colloidal PIC particles. This way, PIC particles containing different Pol-B:PSS ratios have been prepared and their stability under simulated physiological conditions (i.e. pH, osmotic pressure and temperature) characterised. Furthermore, preliminary evaluation of the antimicrobial activity of these Pol-B containing PIC particles has been performed, by monitoring their effect on the growth of Pseudomonas aeruginosa, an opportunistic gram-negative bacterium.

KW - PIC particles

KW - Polyelectrolyte complexes

KW - Antimicrobials

KW - Drug delivery

U2 - 10.1016/j.eurpolymj.2016.08.023

DO - 10.1016/j.eurpolymj.2016.08.023

M3 - Article

VL - 87

SP - 478

EP - 486

JO - European Polymer Journal

JF - European Polymer Journal

SN - 0014-3057

ER -