Prediction of recombinant protein production in an insect cell-baculovirus system using a flow cytometric technique

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Prediction of recombinant protein production in an insect cell-baculovirus system using a flow cytometric technique. / Sandhu, KS; Naciri, M; Al-Rubeai, Mohamed.

In: Journal of Immunological Methods, Vol. 325, No. 1-2, 01.08.2007, p. 104-113.

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@article{98fc6cac0ec64e459de74ddc771e6403,
title = "Prediction of recombinant protein production in an insect cell-baculovirus system using a flow cytometric technique",
abstract = "The baculovirus expression vector system (BEVS) utilising the Autographa californica nucleopolyhedrovirus (AcMNPV) is widely becoming the system of choice for the production of many recombinant protein products due to the high yields obtained. However, there is a need to develop a simple reliable on-line method to monitor the production of recombinant proteins that have no intrinsic reporter properties. Here we utilise flow cytometry to measure cell size, granularity and DNA content in a single step analysis and correlate these parameters with the production of the recombinant protein P-galactosidase. Clear correlations between these parameters and productivity are made with forward and side scatter signals showing the highest correlation coefficients. Measuring these parameters does not require any processing of the cells from culture to analysis. These parameters can therefore be used successfully to predict the amount of recombinant protein product in a BEVS system on-line. (C) 2007 Elsevier B.V. All rights reserved.",
keywords = "recombinant protein, baculovirus, monitoring, insect cells, flow cytometry",
author = "KS Sandhu and M Naciri and Mohamed Al-Rubeai",
year = "2007",
month = aug,
day = "1",
doi = "10.1016/j.jim.2007.06.005",
language = "English",
volume = "325",
pages = "104--113",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Prediction of recombinant protein production in an insect cell-baculovirus system using a flow cytometric technique

AU - Sandhu, KS

AU - Naciri, M

AU - Al-Rubeai, Mohamed

PY - 2007/8/1

Y1 - 2007/8/1

N2 - The baculovirus expression vector system (BEVS) utilising the Autographa californica nucleopolyhedrovirus (AcMNPV) is widely becoming the system of choice for the production of many recombinant protein products due to the high yields obtained. However, there is a need to develop a simple reliable on-line method to monitor the production of recombinant proteins that have no intrinsic reporter properties. Here we utilise flow cytometry to measure cell size, granularity and DNA content in a single step analysis and correlate these parameters with the production of the recombinant protein P-galactosidase. Clear correlations between these parameters and productivity are made with forward and side scatter signals showing the highest correlation coefficients. Measuring these parameters does not require any processing of the cells from culture to analysis. These parameters can therefore be used successfully to predict the amount of recombinant protein product in a BEVS system on-line. (C) 2007 Elsevier B.V. All rights reserved.

AB - The baculovirus expression vector system (BEVS) utilising the Autographa californica nucleopolyhedrovirus (AcMNPV) is widely becoming the system of choice for the production of many recombinant protein products due to the high yields obtained. However, there is a need to develop a simple reliable on-line method to monitor the production of recombinant proteins that have no intrinsic reporter properties. Here we utilise flow cytometry to measure cell size, granularity and DNA content in a single step analysis and correlate these parameters with the production of the recombinant protein P-galactosidase. Clear correlations between these parameters and productivity are made with forward and side scatter signals showing the highest correlation coefficients. Measuring these parameters does not require any processing of the cells from culture to analysis. These parameters can therefore be used successfully to predict the amount of recombinant protein product in a BEVS system on-line. (C) 2007 Elsevier B.V. All rights reserved.

KW - recombinant protein

KW - baculovirus

KW - monitoring

KW - insect cells

KW - flow cytometry

U2 - 10.1016/j.jim.2007.06.005

DO - 10.1016/j.jim.2007.06.005

M3 - Article

C2 - 17643445

VL - 325

SP - 104

EP - 113

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1-2

ER -