Precise Generation of Selective Surface-Confined Glycoprotein Recognition Sites

Research output: Contribution to journalArticlepeer-review

Authors

External organisations

  • University of Birmingham
  • Imperial College London
  • Open University

Abstract

Since glycoproteins have become increasingly recognized as key players in a wide variety of disease processes, there is an increasing need for advanced affinity materials for highly selective glycoprotein binding. Herein, for the first time, a surface-initiated controlled radical polymerization is integrated with supramolecular templating and molecular imprinting to yield highly reproducible synthetic recognition sites on surfaces with dissociation constants (KD) in the low micromolar range for target glycoproteins and minimal binding to nontarget glycoproteins. Importantly, it is shown that the synthetic strategy has a remarkable ability to distinguish the glycosylated and nonglycosylated forms of the same glycoprotein, with a >5-fold difference in binding affinity. The precise control over the polymer film thickness and positioning of multiple carbohydrate receptors plays a crucial role in achieving an enhanced affinity and selectivity. The generated functional materials of unprecedented glycoprotein recognition performance open up a wealth of opportunities in the biotechnological and biomedical fields.

Details

Original languageEnglish
Pages (from-to)2617-2623
Number of pages7
JournalACS Applied Bio Materials
Volume2
Issue number6
Early online date13 May 2019
Publication statusPublished - 17 Jun 2019