Position effects on promoter activity in Escherichia coli and their consequences for antibiotic-resistance determinants

Karen Cooke; Douglas F Browning; David J Lee; Jessica M A Blair; Helen E McNeill; Damon Huber; Stephen J W Busby; Jack A Bryant

doi:10.1042/BST20180503

Position effects on promoter activity in Escherichia coli and their consequences for antibiotic-resistance determinants

Karen Cooke, Douglas F Browning, David J Lee, Jessica M A Blair, Helen E McNeill, Damon Huber, Stephen J W Busby, Jack A Bryant

Research output: Contribution to journal › Review article › peer-review

Abstract

The activity of any bacterial promoter is generally supposed to be set by its base sequence and the different transcription factors that bind in the local vicinity. Here, we review recent data indicating that the activity of the Escherichia coli lac operon promoter also depends upon its chromosomal location. Factors that affect promoter activity include the binding of nucleoid-associated proteins to neighbouring sequences, supercoiling and the activity of neighbouring promoters. We suggest that many bacterial promoters might be susceptible to similar position-dependent effects and we review recent data showing that the expression of mobile genes encoding antibiotic-resistance determinants is also location-dependent, both when carried on a bacterial chromosome or a conjugative plasmid.

Original language	English
Pages (from-to)	839-845
Number of pages	7
Journal	Biochemical Society Transactions
Volume	47
Issue number	3
Early online date	12 Jun 2019
DOIs	https://doi.org/10.1042/BST20180503
Publication status	Published - 28 Jun 2019

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ASJC Scopus subject areas

Biochemistry

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title = "Position effects on promoter activity in Escherichia coli and their consequences for antibiotic-resistance determinants",

abstract = "The activity of any bacterial promoter is generally supposed to be set by its base sequence and the different transcription factors that bind in the local vicinity. Here, we review recent data indicating that the activity of the Escherichia coli lac operon promoter also depends upon its chromosomal location. Factors that affect promoter activity include the binding of nucleoid-associated proteins to neighbouring sequences, supercoiling and the activity of neighbouring promoters. We suggest that many bacterial promoters might be susceptible to similar position-dependent effects and we review recent data showing that the expression of mobile genes encoding antibiotic-resistance determinants is also location-dependent, both when carried on a bacterial chromosome or a conjugative plasmid.",

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AU - Cooke, Karen

AU - Browning, Douglas F

AU - Lee, David J

AU - Blair, Jessica M A

AU - McNeill, Helen E

AU - Huber, Damon

AU - Busby, Stephen J W

AU - Bryant, Jack A

PY - 2019/6/28

Y1 - 2019/6/28

N2 - The activity of any bacterial promoter is generally supposed to be set by its base sequence and the different transcription factors that bind in the local vicinity. Here, we review recent data indicating that the activity of the Escherichia coli lac operon promoter also depends upon its chromosomal location. Factors that affect promoter activity include the binding of nucleoid-associated proteins to neighbouring sequences, supercoiling and the activity of neighbouring promoters. We suggest that many bacterial promoters might be susceptible to similar position-dependent effects and we review recent data showing that the expression of mobile genes encoding antibiotic-resistance determinants is also location-dependent, both when carried on a bacterial chromosome or a conjugative plasmid.

AB - The activity of any bacterial promoter is generally supposed to be set by its base sequence and the different transcription factors that bind in the local vicinity. Here, we review recent data indicating that the activity of the Escherichia coli lac operon promoter also depends upon its chromosomal location. Factors that affect promoter activity include the binding of nucleoid-associated proteins to neighbouring sequences, supercoiling and the activity of neighbouring promoters. We suggest that many bacterial promoters might be susceptible to similar position-dependent effects and we review recent data showing that the expression of mobile genes encoding antibiotic-resistance determinants is also location-dependent, both when carried on a bacterial chromosome or a conjugative plasmid.

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SP - 839

EP - 845

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

IS - 3

ER -

Position effects on promoter activity in Escherichia coli and their consequences for antibiotic-resistance determinants

Abstract

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