Position effects on promoter activity in Escherichia coli and their consequences for antibiotic-resistance determinants
Research output: Contribution to journal › Review article › peer-review
- School of Biosciences, University of Birmingham
- Department of Life Sciences, School of Health Sciences, Birmingham City University, Birmingham B15 3TN, U.K.
The activity of any bacterial promoter is generally supposed to be set by its base sequence and the different transcription factors that bind in the local vicinity. Here, we review recent data indicating that the activity of the Escherichia coli lac operon promoter also depends upon its chromosomal location. Factors that affect promoter activity include the binding of nucleoid-associated proteins to neighbouring sequences, supercoiling and the activity of neighbouring promoters. We suggest that many bacterial promoters might be susceptible to similar position-dependent effects and we review recent data showing that the expression of mobile genes encoding antibiotic-resistance determinants is also location-dependent, both when carried on a bacterial chromosome or a conjugative plasmid.
|Number of pages||7|
|Journal||Biochemical Society Transactions|
|Early online date||12 Jun 2019|
|Publication status||Published - 28 Jun 2019|