Pneumolysin activates the synthesis and release of interleukin-8 by human neutrophils in vitro

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Pneumolysin activates the synthesis and release of interleukin-8 by human neutrophils in vitro. / Cockeran, Riana; Durandt, Chrisna; Feldman, Charles; Mitchell, Timothy J; Anderson, Ronald.

In: The Journal of Infectious Diseases, Vol. 186, No. 4, 15.08.2002, p. 562-5.

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Cockeran, Riana ; Durandt, Chrisna ; Feldman, Charles ; Mitchell, Timothy J ; Anderson, Ronald. / Pneumolysin activates the synthesis and release of interleukin-8 by human neutrophils in vitro. In: The Journal of Infectious Diseases. 2002 ; Vol. 186, No. 4. pp. 562-5.

Bibtex

@article{12cf396a738e41b691367ae4e03aec9a,
title = "Pneumolysin activates the synthesis and release of interleukin-8 by human neutrophils in vitro",
abstract = "The effects that the Streptococcus pneumoniae-derived, proinflammatory toxin, pneumolysin (8.37 and 41.75 ng/mL), has on the production of interleukin (IL)-8 and tumor-necrosis factor (TNF)-alpha by human neutrophils have been investigated in vitro. Total and extracellular IL-8 and TNF-alpha were assayed by enzyme-linked immunosorbent assay, and flow cytometry and colorimetric procedures were used to detect intracellular cytokine and cytokine messenger RNA, respectively. Treatment of neutrophils with pneumolysin either alone or in combination with the chemotactic tripeptide, N-formyl-l-methionyl-l-leucyl-l-phenylalanine (1 microM), resulted in a time-dependent (maximal at 6 h) increase in synthesis and release of IL-8 but not of TNF-alpha, which was associated with increased expression of IL-8 messenger RNA transcripts and was abrogated by either cycloheximide (10 microg/mL) or depletion of Ca(2+) from the cell-suspending medium. These interactions between the toxin and neutrophils may contribute to the exaggerated pulmonary inflammatory responses caused by pneumolysin-producing strains of the pneumococcus.",
keywords = "Bacterial Proteins, Cells, Cultured, Humans, Interleukin-8, N-Formylmethionine Leucyl-Phenylalanine, Neutrophil Activation, Neutrophils, RNA, Messenger, Recombinant Proteins, Streptococcus pneumoniae, Streptolysins, Tumor Necrosis Factor-alpha",
author = "Riana Cockeran and Chrisna Durandt and Charles Feldman and Mitchell, {Timothy J} and Ronald Anderson",
year = "2002",
month = aug,
day = "15",
doi = "10.1086/341563",
language = "English",
volume = "186",
pages = "562--5",
journal = "The Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "4",

}

RIS

TY - JOUR

T1 - Pneumolysin activates the synthesis and release of interleukin-8 by human neutrophils in vitro

AU - Cockeran, Riana

AU - Durandt, Chrisna

AU - Feldman, Charles

AU - Mitchell, Timothy J

AU - Anderson, Ronald

PY - 2002/8/15

Y1 - 2002/8/15

N2 - The effects that the Streptococcus pneumoniae-derived, proinflammatory toxin, pneumolysin (8.37 and 41.75 ng/mL), has on the production of interleukin (IL)-8 and tumor-necrosis factor (TNF)-alpha by human neutrophils have been investigated in vitro. Total and extracellular IL-8 and TNF-alpha were assayed by enzyme-linked immunosorbent assay, and flow cytometry and colorimetric procedures were used to detect intracellular cytokine and cytokine messenger RNA, respectively. Treatment of neutrophils with pneumolysin either alone or in combination with the chemotactic tripeptide, N-formyl-l-methionyl-l-leucyl-l-phenylalanine (1 microM), resulted in a time-dependent (maximal at 6 h) increase in synthesis and release of IL-8 but not of TNF-alpha, which was associated with increased expression of IL-8 messenger RNA transcripts and was abrogated by either cycloheximide (10 microg/mL) or depletion of Ca(2+) from the cell-suspending medium. These interactions between the toxin and neutrophils may contribute to the exaggerated pulmonary inflammatory responses caused by pneumolysin-producing strains of the pneumococcus.

AB - The effects that the Streptococcus pneumoniae-derived, proinflammatory toxin, pneumolysin (8.37 and 41.75 ng/mL), has on the production of interleukin (IL)-8 and tumor-necrosis factor (TNF)-alpha by human neutrophils have been investigated in vitro. Total and extracellular IL-8 and TNF-alpha were assayed by enzyme-linked immunosorbent assay, and flow cytometry and colorimetric procedures were used to detect intracellular cytokine and cytokine messenger RNA, respectively. Treatment of neutrophils with pneumolysin either alone or in combination with the chemotactic tripeptide, N-formyl-l-methionyl-l-leucyl-l-phenylalanine (1 microM), resulted in a time-dependent (maximal at 6 h) increase in synthesis and release of IL-8 but not of TNF-alpha, which was associated with increased expression of IL-8 messenger RNA transcripts and was abrogated by either cycloheximide (10 microg/mL) or depletion of Ca(2+) from the cell-suspending medium. These interactions between the toxin and neutrophils may contribute to the exaggerated pulmonary inflammatory responses caused by pneumolysin-producing strains of the pneumococcus.

KW - Bacterial Proteins

KW - Cells, Cultured

KW - Humans

KW - Interleukin-8

KW - N-Formylmethionine Leucyl-Phenylalanine

KW - Neutrophil Activation

KW - Neutrophils

KW - RNA, Messenger

KW - Recombinant Proteins

KW - Streptococcus pneumoniae

KW - Streptolysins

KW - Tumor Necrosis Factor-alpha

U2 - 10.1086/341563

DO - 10.1086/341563

M3 - Article

C2 - 12195386

VL - 186

SP - 562

EP - 565

JO - The Journal of Infectious Diseases

JF - The Journal of Infectious Diseases

SN - 0022-1899

IS - 4

ER -