Platelets secrete stromal cell-derived factor 1  and recruit bone marrow-derived progenitor cells to arterial thrombi in vivo

Research output: Contribution to journalArticle

Authors

  • S Massberg
  • I Konrad
  • K Schürzinger
  • M Lorenz
  • S Schneider
  • D Zohlnhoefer
  • K Hoppe
  • M Schiemann
  • E Kennerknecht
  • S Sauer
  • C Schulz
  • S Kerstan
  • M Rudelius
  • S Seidl
  • F Sorge
  • H Langer
  • M Peluso
  • P Goyal
  • D Vestweber
  • NR Emambokus

Abstract

The accumulation of smooth muscle and endothelial cells is essential for remodeling and repair of injured blood vessel walls. Bone marrow-derived progenitor cells have been implicated in vascular repair and remodeling; however, the mechanisms underlying their recruitment to the site of injury remain elusive. Here, using real-time in vivo fluorescence microscopy, we show that platelets provide the critical signal that recruits CD34+ bone marrow cells and c-Kit+ Sca-1+ Lin- bone marrow-derived progenitor cells to sites of vascular injury. Correspondingly, specific inhibition of platelet adhesion virtually abrogated the accumulation of both CD34+ and c-Kit+ Sca-1+ Lin- bone marrow-derived progenitor cells at sites of endothelial disruption. Binding of bone marrow cells to platelets involves both P-selectin and GPIIb integrin on platelets. Unexpectedly, we found that activated platelets secrete the chemokine SDF-1alpha, thereby supporting further primary adhesion and migration of progenitor cells. These findings establish the platelet as a major player in the initiation of vascular remodeling, a process of fundamental importance for vascular repair and pathological remodeling after vascular injury.

Details

Original languageEnglish
Pages (from-to)1221-33
Number of pages13
JournalThe Journal of Experimental Medicine
Volume203
Issue number5
Early online date17 Apr 2006
Publication statusPublished - 17 Apr 2006