Platelet actin nodules are podosome-like structures dependent on Wiskott-Aldrich Syndrome protein and ARP2/3 complex

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@article{a1fd39bb53b349129f980ee316bd800e,
title = "Platelet actin nodules are podosome-like structures dependent on Wiskott-Aldrich Syndrome protein and ARP2/3 complex",
abstract = "The actin nodule is a novel F-actin structure present in platelets during early spreading. However, only limited detail is known regarding nodule organisation and function. Here we use electron-microscopy, SIM and dSTORM super-resolution, and live-cell TIRF microscopy to characterise the structural organisation and signalling pathways associated with nodule formation. Nodules are composed of up to 4 actin-rich structures linked together by actin bundles. They are enriched in the adhesion-related proteins talin and vinculin, have a central core of tyrosine phosphorylated proteins and are depleted of integrins at the plasma membrane. Nodule formation is dependent on Wiskott-Aldrich Syndrome protein (WASp) and the ARP2/3 complex. WASp-/-mouse blood displays impaired platelet aggregate formation at arteriolar shear rates. We propose actin nodules are platelet podosome-related structures required for platelet-platelet interaction and their absence contributes to the bleeding diathesis of Wiskott-Aldrich Syndrome. ",
keywords = "Biological sciences, Cell biology",
author = "Natalie Poulter and Alice Pollitt and Amy Davies and Dessislava Malinova and Joshua Rappoport and Gerard Nash and Michael Hannon and Zoe Pikramenou and Hartwig, {John H} and Thrasher, {Adrian J} and Steve Watson and Steven Thomas and Dylan Owen",
year = "2015",
month = jun,
day = "1",
doi = "10.1038/ncomms8254",
language = "English",
volume = "6",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - Platelet actin nodules are podosome-like structures dependent on Wiskott-Aldrich Syndrome protein and ARP2/3 complex

AU - Poulter, Natalie

AU - Pollitt, Alice

AU - Davies, Amy

AU - Malinova, Dessislava

AU - Rappoport, Joshua

AU - Nash, Gerard

AU - Hannon, Michael

AU - Pikramenou, Zoe

AU - Hartwig, John H

AU - Thrasher, Adrian J

AU - Watson, Steve

AU - Thomas, Steven

AU - Owen, Dylan

PY - 2015/6/1

Y1 - 2015/6/1

N2 - The actin nodule is a novel F-actin structure present in platelets during early spreading. However, only limited detail is known regarding nodule organisation and function. Here we use electron-microscopy, SIM and dSTORM super-resolution, and live-cell TIRF microscopy to characterise the structural organisation and signalling pathways associated with nodule formation. Nodules are composed of up to 4 actin-rich structures linked together by actin bundles. They are enriched in the adhesion-related proteins talin and vinculin, have a central core of tyrosine phosphorylated proteins and are depleted of integrins at the plasma membrane. Nodule formation is dependent on Wiskott-Aldrich Syndrome protein (WASp) and the ARP2/3 complex. WASp-/-mouse blood displays impaired platelet aggregate formation at arteriolar shear rates. We propose actin nodules are platelet podosome-related structures required for platelet-platelet interaction and their absence contributes to the bleeding diathesis of Wiskott-Aldrich Syndrome.

AB - The actin nodule is a novel F-actin structure present in platelets during early spreading. However, only limited detail is known regarding nodule organisation and function. Here we use electron-microscopy, SIM and dSTORM super-resolution, and live-cell TIRF microscopy to characterise the structural organisation and signalling pathways associated with nodule formation. Nodules are composed of up to 4 actin-rich structures linked together by actin bundles. They are enriched in the adhesion-related proteins talin and vinculin, have a central core of tyrosine phosphorylated proteins and are depleted of integrins at the plasma membrane. Nodule formation is dependent on Wiskott-Aldrich Syndrome protein (WASp) and the ARP2/3 complex. WASp-/-mouse blood displays impaired platelet aggregate formation at arteriolar shear rates. We propose actin nodules are platelet podosome-related structures required for platelet-platelet interaction and their absence contributes to the bleeding diathesis of Wiskott-Aldrich Syndrome.

KW - Biological sciences

KW - Cell biology

U2 - 10.1038/ncomms8254

DO - 10.1038/ncomms8254

M3 - Article

C2 - 26028144

VL - 6

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 7254

ER -